Neurosurgical Department, Brain and Nerve Research Laboratory, The First Affiliated Hospital of Soochow University, 188 Shizi Street, 215006, Suzhou, China.
J Exp Clin Cancer Res. 2010 Jun 29;29(1):84. doi: 10.1186/1756-9966-29-84.
Orthotopic models are important in cancer research. Here we developed orthotopic xenograft mouse model of metastatic lung cancer and glioblastoma with a specially designed system.
Tiny fragments of surgical tumors were implanted into the mice brain with a trocar system. Immunohistochemistry was performed to detect brain tumor stem cells among glioblastoma tissues, including both the original and resulting ones with monoclonal antibody against CD133.
Besides the constant high take rates in both models; brain transplants perfectly resembled their original tumors in biological behaviors. The brain tumor stem cells, positively stained with CD133 were found, though not frequently, in both original and resulting glioblastoma tissues.
Orthotopic model established with a trocar system is effective and injection of tumor tissues containing stem cells promise the forming of new tumor mass when grafted.
原位模型在癌症研究中很重要。 在这里，我们使用专门设计的系统开发了转移性肺癌和神经胶质瘤的原位异种移植小鼠模型。
使用套管系统将手术肿瘤的微小碎片植入小鼠大脑中。 进行免疫组织化学染色，以检测神经胶质瘤组织中的脑肿瘤干细胞，包括针对 CD133 的单克隆抗体的原始和衍生肿瘤。
除了两种模型的恒定高吸收率外；脑移植在生物学行为上与原始肿瘤完全相似。 虽然并不常见，但在原始和衍生的神经胶质瘤组织中都发现了 CD133 阳性染色的脑肿瘤干细胞。
套管系统建立的原位模型是有效的，并且当移植时，含有干细胞的肿瘤组织的注射有望形成新的肿瘤团块。
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