UR 09/30: Genetic and Biodiversity, Institute of Biotechnology, 5000 Monastir, Tunisia.
J Environ Sci (China). 2009;21(11):1620-4. doi: 10.1016/s1001-0742(08)62464-x.
The present study tested the sensitivity of Salaria basilisca to water-cadmium (Cd) contamination. For this purpose, liver somatic index (LSI), Cd concentrations and the activities of antioxidant enzymes such as catalase (CAT), superoxide dismutase (SOD) and glutathione peroxidase (GSH-Px) were measured in the liver of S. basilisca exposed to Cd-contaminated water (2 mg Cd/L as CdCl2) for 14 and 28 d. The results showed that the LSI decreased significantly after 14 and 28 d of Cd-exposure. Cd bioaccumulation in the liver resulted in an increasing uptake up to 42 microg/g dry weight after 28 d of exposure. Activities of CAT and SOD were significantly increased with increasing exposure time. A significant increase in GSH-Px activity, under Cd influence, was observed during 14-day exposure period (p < 0.0001). However, a significant decrease (p < 0.05) in this activity with respect to control fish was registered after 28 d of Cd-exposure. These results showed that Cd accumulation in the liver of S. basilisca could induce oxidative stress as demonstrated by changes in the antioxidant enzyme activities. Results also emphasized that S. basilisca may considered as a sensitive species to Cd exposure.
本研究旨在测试巴沙鱼对水镉（Cd）污染的敏感性。为此，我们测量了暴露在 Cd 污染水中（以 CdCl2 的形式存在，浓度为 2mg/L）14 天和 28 天的巴沙鱼肝脏中的肝体指数（LSI）、Cd 浓度以及抗氧化酶（如 CAT、SOD 和 GSH-Px）的活性。结果表明，暴露 14 天和 28 天后，LSI 显著降低。肝组织中的 Cd 生物积累导致暴露 28 天后的吸收量增加至 42μg/g 干重。CAT 和 SOD 的活性随着暴露时间的增加而显著增加。在 14 天的暴露期内，GSH-Px 活性显著增加（p＜0.0001）。然而，暴露 28 天后，与对照组相比，该活性显著下降（p＜0.05）。这些结果表明，Cd 在巴沙鱼肝脏中的积累可能会导致氧化应激，这可以通过抗氧化酶活性的变化来证明。研究结果还强调，巴沙鱼可能被认为是对 Cd 暴露敏感的物种。
