Heese Traci, Jenkinson Jack, Love Cheryl, Milam Ronald, Perkins Lillian, Adams Cynthia, McCall Suzanne, Ceremuga Thomas E
US Army Graduate Program in Anesthesia Nursing, Carl R. Darnell Army Medical Center, Fort Hood, Texas, USA.
AANA J. 2009 Dec;77(6):445-9.
The purpose of the study was to investigate the anxiolytic effects of L-theanine and its potential interaction with the GABAA receptor in Sprague-Dawley rats. L-theanine is a major component of green tea, which has traditionally been used as an herbal remedy in the treatment of many medical conditions, including anxiety. Herbals and supplements and their potential interactions perioperatively are a concern to anesthetists. Fifty-five rats were divided into 5 groups: control (saline); L-theanine (positive control); flumazenil (a known benzodiazepine receptor antagonist) and L-theanine; and midazolam and L-theanine. The behavioral component of anxiety was evaluated using the elevated plus-maze and calculated by the time spent in the open arm of the maze divided by total time in the maze. Data were analyzed using a 2-tailed multivariate analysis of variance and Sheffé posthoc test. The data suggest that L-theanine does not produce anxiolysis by modulation of the GABAA receptor; however, in combination with midazolam, a synergistic or additive effect was demonstrated by decreased anxiety and both fine and basic motor movements. These data may provide direction for further studies examining L-theanine and its effects on anxiety and motor activity.
该研究的目的是调查L-茶氨酸在斯普拉格-道利大鼠中的抗焦虑作用及其与GABAA受体的潜在相互作用。L-茶氨酸是绿茶的主要成分，传统上被用作草药治疗包括焦虑在内的多种疾病。草药和补充剂及其围手术期的潜在相互作用是麻醉医生关注的问题。55只大鼠被分为5组：对照组（生理盐水）；L-茶氨酸组（阳性对照）；氟马西尼（一种已知的苯二氮䓬受体拮抗剂）和L-茶氨酸组；咪达唑仑和L-茶氨酸组。使用高架十字迷宫评估焦虑的行为成分，并通过大鼠在迷宫开放臂所花费的时间除以在迷宫中的总时间来计算。数据采用双尾多因素方差分析和谢费尔事后检验进行分析。数据表明，L-茶氨酸不是通过调节GABAA受体产生抗焦虑作用；然而，与咪达唑仑联合使用时，通过降低焦虑以及精细和基本运动活动，显示出协同或相加作用。这些数据可能为进一步研究L-茶氨酸及其对焦虑和运动活动的影响提供方向。
