[2008年从甘肃省分离的日本脑炎病毒的分子特征]
[Molecular characterization of Japanese encephalitis virus isolated from Gansu province in 2008].
作者信息
Li Ming-Hua, Fu Shi-Hong, Feng Yun, Gao Xiao-Yan, Zhai You-Gang, Yu De-Shan, Li Guo-Tai, Jia Yu-Xin, Liang Guo-Dong
机构信息
State Key Laboratory for Infectious Disease Prevention and Control, Institute for Viral Disease Control and Prevention, Chinese Center for Disease Control and Prevention, Beijing 100052, China.
出版信息
Zhonghua Shi Yan He Lin Chuang Bing Du Xue Za Zhi. 2009 Aug;23(4):251-3.
OBJECTIVE
To sequence PrM and E gene of the Japanese encephalitis virus isolated from Gansu province in 2008 and analysis the genotype of new JEV isolates and the molecular characterization of E gene.
METHODS
Computer software was used to analyze nucleic acid sequence and deduced amino acid sequence, and draw phylogenetic trees, including ClustalX2.09, MegAlign and Mega4.
RESULTS
The six JEV strains were clustered in genotype I. 87.5%-87.9% identity in nucleotide sequence and 96.8%-97.2% identity in amino acid sequence were found in E gene when compared with the vaccine strain SA14-14-2. Eleven common amino acid differences were observed in E protein between new isolates and the vaccine strain.
CONCLUSION
Genotype I JEVs were isolated from mosquitoes collected in Gansu province. The amino acid difference occurred in sites that were not the key ones affecting the antigenic of JEV.
目的
对2008年从甘肃省分离的乙型脑炎病毒的前膜蛋白（PrM）和包膜蛋白（E）基因进行测序，分析新分离的乙型脑炎病毒基因型及E基因的分子特征。
方法
利用计算机软件分析核酸序列和推导的氨基酸序列，并绘制系统发育树，所用软件包括ClustalX2.09、MegAlign和Mega4。
结果
6株乙型脑炎病毒株聚类于基因型I。与疫苗株SA14-14-2相比，E基因核苷酸序列的同源性为87.5% - 87.9%，氨基酸序列的同源性为96.8% - 97.2%。新分离株与疫苗株的E蛋白之间观察到11个常见的氨基酸差异。
结论
从甘肃省采集的蚊虫中分离到基因型I的乙型脑炎病毒。氨基酸差异发生在对乙型脑炎病毒抗原性无关键影响的位点。
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