Chen Duan, Wang Huan-yu, Fu Shi-hong, Song Hong, Deng Juan, Yang Yi-liang, Liang Guo-dong
State Key Laboratory for Molecular Virology, Fujian Center for Disease Control and Prevention, Fuzhou 350001, China.
Zhonghua Shi Yan He Lin Chuang Bing Du Xue Za Zhi. 2005 Mar;19(1):5-8.
To analyze the genotype of newly isolated Japanese encephalitis viruses in Fujian province and the characteristics of amino acid sequence in the E gene.
PrM and E segments of newly isolated Japanese encephalitis viruses were amplified by RT-PCR, the PCR products were cloned into T vector for sequencing. Phylogenetic analysis was carried out by PHYLIP program.
Newly isolated Japanese encephalitis viruses belonged to genotype III, the nucleotide and amino acid of E gene showed high homology to vaccine strain SA14-14-2, but had some difference in some domains.
Newly isolated viruses in Fujian province belonged to Japanese encephalitis virus genotype III. E protein of the isolates showed some differences as compared with vaccine strains.
分析福建省新分离的乙型脑炎病毒的基因型及E基因氨基酸序列特征。
采用RT-PCR扩增新分离乙型脑炎病毒的PrM和E片段，将PCR产物克隆至T载体进行测序。用PHYLIP程序进行系统发育分析。
新分离的乙型脑炎病毒属于Ⅲ型基因型，E基因核苷酸和氨基酸与疫苗株SA14-14-2具有高度同源性，但在某些区域存在差异。
福建省新分离的病毒属于乙型脑炎病毒Ⅲ型基因型。分离株的E蛋白与疫苗株相比存在一些差异。
