[福建省三株日本脑炎病毒新分离株的分子特征]
[Molecular characteristics of three new isolates of Japanese encephalitis virus in Fujian Province].
作者信息
Chen Duan, Wang Huan-yu, Fu Shi-hong, Song Hong, Deng Juan, Yang Yi-liang, Liang Guo-dong
机构信息
State Key Laboratory for Molecular Virology, Fujian Center for Disease Control and Prevention, Fuzhou 350001, China.
出版信息
Zhonghua Shi Yan He Lin Chuang Bing Du Xue Za Zhi. 2005 Mar;19(1):5-8.
OBJECTIVE
To analyze the genotype of newly isolated Japanese encephalitis viruses in Fujian province and the characteristics of amino acid sequence in the E gene.
METHODS
PrM and E segments of newly isolated Japanese encephalitis viruses were amplified by RT-PCR, the PCR products were cloned into T vector for sequencing. Phylogenetic analysis was carried out by PHYLIP program.
RESULTS
Newly isolated Japanese encephalitis viruses belonged to genotype III, the nucleotide and amino acid of E gene showed high homology to vaccine strain SA14-14-2, but had some difference in some domains.
CONCLUSION
Newly isolated viruses in Fujian province belonged to Japanese encephalitis virus genotype III. E protein of the isolates showed some differences as compared with vaccine strains.
目的
分析福建省新分离的乙型脑炎病毒的基因型及E基因氨基酸序列特征。
方法
采用RT-PCR扩增新分离乙型脑炎病毒的PrM和E片段，将PCR产物克隆至T载体进行测序。用PHYLIP程序进行系统发育分析。
结果
新分离的乙型脑炎病毒属于Ⅲ型基因型，E基因核苷酸和氨基酸与疫苗株SA14-14-2具有高度同源性，但在某些区域存在差异。
结论
福建省新分离的病毒属于乙型脑炎病毒Ⅲ型基因型。分离株的E蛋白与疫苗株相比存在一些差异。
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