Huang Lei, Zhou Xian-Zhi, Zhao Jing-Min, Wu Hao, Jin Rui, Meng Xin, Pang Deng, Zhang Shi-Jie
PLA 302 Hospital, Beijing 100039, China
Zhonghua Shi Yan He Lin Chuang Bing Du Xue Za Zhi. 2009 Aug;23(4):261-4.
To investigate the frequencies and distribution of CD4+ T cells and CD8+ T cells as well as the changes of immune activation in gastric mucosa of HIV-infected individuals.
42 HIV-infected individuals were recruited into this investigation, and 36 patients had definite diagnosis of clinical stage. Biopsy of gastric mucosal tissues was performed by fiberoptic gastroscope including 10 normal people as a control group. Then, immunohistochemistry was used to detect expression of CD4, CD8 and CD38 in gastric mucosa, and the distinctions among three groups were analyzed with LEICA Qwin image analysis system.
(1) Compared with asymptomatic HIV carriers and control group, CD4 T cells remarkably decreased in the gastric mucosa of AIDS patients (P < 0.01). In gastric mucosa of asymptomatic HIV carriers, there were still some CD4+ T cells in lymphoid follicles and stroma where CD4+ T cells were unevenly distributed, the frequency of CD4+ T cells was not significantly different between asymptomatic HIV carriers and control group (P > 0.05); (2) Phenomenon of CD8+ T cells infiltrating mucosal epithelium and gland was general in HIV-infected individuals. CD8+ T cells took on local excessive hyperplasia in gastric mucosa of some individuals. As compared with control group, CD8+ T cells markedly increased in gastric mucosa of infected individuals (P < 0.01), but the distinction of asymptomatic HIV carriers and AIDS patients was not significant (P > 0.05); (3) CD38-expressing cells mainly distributed over gastric mucosal surface to superficial layer(1/3-2/3 layer) of HIV-infected individuals, and was more intensive than control group (P < or = 0.01), but there was not noticeable difference between asymptomatic HIV carriers and AIDS patients (P > 0.05).
The frequencies and distribution of gastric mucosal CD4+ T cells of HIV-infected individuals were closely correlated with progression of disease. Disfunction of mucosal immune system which was resulted from HIV infection and injury of CD4+ T cells could be an important cause of CD8+ T cells increasing and CD38-expressing enhancement.
探讨HIV感染者胃黏膜中CD4+T细胞和CD8+T细胞的频率及分布情况，以及免疫激活的变化。
招募42例HIV感染者参与本研究，其中36例患者临床分期明确。通过纤维胃镜对胃黏膜组织进行活检，包括10名正常人作为对照组。然后，采用免疫组织化学法检测胃黏膜中CD4、CD8和CD38的表达，并使用徕卡Qwin图像分析系统分析三组之间的差异。
（1）与无症状HIV携带者和对照组相比，艾滋病患者胃黏膜中的CD4 T细胞显著减少（P<0.01）。在无症状HIV携带者的胃黏膜中，淋巴滤泡和基质中仍有一些CD4+T细胞，且CD4+T细胞分布不均，无症状HIV携带者与对照组之间CD4+T细胞的频率无显著差异（P>0.05）；（2）CD8+T细胞浸润黏膜上皮和腺体的现象在HIV感染者中普遍存在。部分个体胃黏膜中CD8+T细胞呈现局部过度增生。与对照组相比，感染个体胃黏膜中的CD8+T细胞显著增加（P<0.01），但无症状HIV携带者与艾滋病患者之间的差异不显著（P>0.05）；（3）表达CD38的细胞主要分布在HIV感染者胃黏膜表面至浅层（1/3 - 2/3层），且比对照组更密集（P≤0.01），但无症状HIV携带者与艾滋病患者之间无明显差异（P>0.05）。
HIV感染者胃黏膜CD4+T细胞的频率和分布与疾病进展密切相关。HIV感染导致的黏膜免疫系统功能障碍以及CD4+T细胞损伤可能是CD8+T细胞增加和CD38表达增强的重要原因。
