Siroy Axel, Cosette Pascal, Seyer Damien, Lemaître-Guillier Christelle, Vallenet David, Van Dorsselaer Alain, Boyer-Mariotte Sophie, Jouenne Thierry, Dé Emmanuelle
IBBR Group, Laboratory Polymères, Biopolymères, Membranes, UMR 6522 CNRS, University of Rouen, France.
J Proteome Res. 2006 Dec;5(12):3385-98. doi: 10.1021/pr060372s.
Acinetobacter baumannii causes severe infections in compromised patients. We combined SDS-PAGE, two-dimensional gel electrophoresis and mass spectrometry (LC-MS/MS and MALDI-TOF) to separate and characterize the proteins of the cell envelope of this bacterium. In total, 135 proteins (inner and outer membrane proteins) were identified. In this analysis, we described the expression by this bacterium of RND-type efflux systems and some potential virulence factors. We then compared the membrane subproteome of a clinical multidrug-resistant (MDR) isolate with that of a reference strain. We found that the MDR strain expressed lower levels of the penicillin-binding-protein 1b, produced a CarO protein having different primary and quaternary structures to that of the reference strain, and expressed OmpW isoforms. We also showed that the clinical strain has a high ability to form biofilms consistent with the accumulation of some outer membrane proteins (OMPs) such as NlpE or CsuD that have already been described as involved in bacterial adhesion. These features may partly explain the MDR emergence of the clinical isolate.
鲍曼不动杆菌可在免疫功能低下的患者中引起严重感染。我们结合了SDS-PAGE、二维凝胶电泳和质谱分析（液相色谱-串联质谱和基质辅助激光解吸电离飞行时间质谱）来分离和鉴定该细菌细胞膜的蛋白质。总共鉴定出135种蛋白质（内膜和外膜蛋白）。在该分析中，我们描述了该细菌对RND型外排系统和一些潜在毒力因子的表达。然后，我们将临床多药耐药（MDR）分离株的膜亚蛋白质组与参考菌株的膜亚蛋白质组进行了比较。我们发现，MDR菌株中青霉素结合蛋白1b的表达水平较低，产生的CarO蛋白与参考菌株的一级和四级结构不同，并表达OmpW同工型。我们还表明，临床菌株具有很强的形成生物膜的能力，这与一些外膜蛋白（OMPs）如NlpE或CsuD的积累一致，这些蛋白已被描述为参与细菌黏附。这些特征可能部分解释了临床分离株的多药耐药性产生的原因。
