Division of Pathological Sciences, Institute of Comparative Medicine, Faculty of Veterinary Medicine, University of Glasgow, Bearsden Road, Garscube Campus, Glasgow, Scotland G61 1QH, UK.
Virus Res. 2010 Apr;149(1):124-7. doi: 10.1016/j.virusres.2010.01.008. Epub 2010 Jan 28.
BPV-1 and less commonly BPV-2 are associated with the pathogenesis of equine skin tumours termed sarcoids. We recently documented the transcriptional changes that are induced by BPV-1 in equine fibroblasts using microarray analyses. TLR4 expression was found to be significantly down-regulated by BPV-1. In the present study, we show that TLR4 expression is significantly decreased following the exogenous expression of BPV-1 E2 and E7 in primary equine fibroblasts. The results were confirmed by the demonstration of increased TLR4 expression following siRNA suppression of BPV-1 E2 and E7 viral gene expression. These data imply that BPV-1 is able to subvert the innate immune response by downregulation of TLR4.
BPV-1 及较少见的 BPV-2 与马的皮肤肿瘤（称为肉瘤）的发病机制有关。我们最近使用微阵列分析记录了 BPV-1 在马成纤维细胞中诱导的转录变化。发现 TLR4 的表达被 BPV-1 显著下调。在本研究中，我们表明在原代马成纤维细胞中外源表达 BPV-1 E2 和 E7 后，TLR4 的表达显著降低。通过 siRNA 抑制 BPV-1 E2 和 E7 病毒基因表达后 TLR4 表达增加证实了这一结果。这些数据表明，BPV-1 通过下调 TLR4 能够颠覆先天免疫反应。
