MRC-University of Glasgow Centre for Virus Research, Institute of Infection, Inflammation and Immunity, College of Medical, Veterinary and Life Sciences, University of Glasgow, Bearsden Road, Glasgow, Scotland, G61 1QH, United Kingdom.
Vet Res. 2012 Dec 4;43(1):81. doi: 10.1186/1297-9716-43-81.
Bovine papillomavirus type 1 infects not only cattle but also equids and is a causative factor in the pathogenesis of commonly occurring equine sarcoid tumours. Whilst treatment of sarcoids is notoriously difficult, cisplatin has been shown to be one of the most effective treatment strategies for sarcoids. In this study we show that in equine fibroblasts, BPV-1 sensitises cells to cisplatin-induced and UVB-induced apoptosis, a known cofactor for papillomavirus associated disease, however BPV-1 transformed fibroblasts show increased clonogenic survival, which may potentially limit the therapeutic effects of repeated cisplatin treatment. Furthermore we show that BPV-1 increases p53 expression in sarcoid cell lines and p53 expression can be either nuclear or cytoplasmic. The mechanism and clinical significance of increase/abnormal p53 expression remains to be established.
牛乳头瘤病毒 1 型不仅感染牛，还感染马属动物，是常见马属动物肉瘤肿瘤发病机制的一个致病因素。虽然肉瘤的治疗众所周知非常困难，但顺铂已被证明是治疗肉瘤最有效的策略之一。在这项研究中，我们表明在马属成纤维细胞中，BPV-1 使细胞对顺铂诱导和 UVB 诱导的细胞凋亡敏感，这是一种已知的与乳头瘤病毒相关疾病的共同作用因子，然而 BPV-1 转化的成纤维细胞显示出增加的集落形成存活能力，这可能会限制重复顺铂治疗的治疗效果。此外，我们表明 BPV-1 增加了肉瘤细胞系中的 p53 表达，并且 p53 表达可以是核内或细胞质内的。p53 表达增加/异常的机制和临床意义仍有待确定。
