Biomaterials and Biomedical Engineering Research Laboratory, Center for Structural and Functional Materials, University of Louisiana at Lafayette, PO Box 44130, Lafayette, LA 70504, USA.
Acta Biomater. 2010 Aug;6(8):3339-48. doi: 10.1016/j.actbio.2010.01.034. Epub 2010 Jan 28.
Given the need to develop high strength/weight ratio bioimplants with enhanced cellular response, we describe here a study focused on the processing-structure-functional property relationship in austenitic stainless steel that was processed using an ingenious phase reversion approach to obtain an nanograined/ultrafine-grained (NG/UFG) structure. The cellular activity between fibroblast and NG/UFG substrate is compared with the coarse-grained (CG) substrate. A comparative investigation of NG/UFG and CG structures illustrated that cell attachment, proliferation, viability, morphology and spread are favorably modulated and significantly different from the conventional CG structure. These observations were further confirmed by expression levels of vinculin and associated actin cytoskeleton. Immunofluorescence studies demonstrated increased vinculin concentrations associated with actin stress fibers in the outer regions of the cells and cellular extensions on NG/UFG substrate. These observations suggest enhanced cell-substrate interaction and activity. The cellular attachment response on NG/UFG substrate is attributed to grain size and hydrophilicity and is related to more open lattice in the positions of high-angle grain boundaries.
鉴于需要开发具有增强细胞反应的高强度/重量比生物植入物，我们在这里描述了一项专注于奥氏体不锈钢的处理-结构-功能关系的研究，该研究采用巧妙的相反转方法获得了纳米/超细晶粒（NG/UFG）结构。比较了成纤维细胞与 NG/UFG 基底之间的细胞活性与粗晶粒（CG）基底。NG/UFG 和 CG 结构的比较研究表明，细胞附着、增殖、活力、形态和扩散得到了有利的调节，与传统的 CG 结构有显著差异。这些观察结果进一步通过 vinculin 的表达水平及其相关的肌动蛋白细胞骨架得到证实。免疫荧光研究表明，在 NG/UFG 基底上，细胞外区域的 vinculin 浓度与肌动蛋白应力纤维增加，细胞突起也增加。这些观察表明增强了细胞-基底相互作用和活性。NG/UFG 基底上的细胞附着反应归因于晶粒尺寸和润湿性，并且与高角度晶界位置的更开放晶格有关。
