Biomaterials and Biomedical Engineering Research Laboratory, Center for Structural and Functional Materials, University of Louisiana at Lafayette, Lafayette, Louisiana 70504, USA.
J Biomed Mater Res A. 2013 Jan;101(1):1-12. doi: 10.1002/jbm.a.34105. Epub 2012 May 8.
The rapid adsorption of proteins is the starting and primary biological response that occurs when a biomedical device is implanted in the physiological system. The biological response, however, depends on the surface characteristics of the device. Considering the significant interest in nano-/ultrafine surfaces and nanostructured coatings, we describe here, the interplay between grain structure and protein adsorption (bovine serum albumin: BSA) on osteoblasts functions by comparing nanograined/ultrafine-grained (NG/UFG) and coarse-grained (CG: grain size in the micrometer range) substrates by investigating cell-substrate interactions. The protein adsorption on NG/UFG surface was beneficial in favorably modulating biological functions including cell attachment, proliferation, and viability, whereas the effect was less pronounced on protein adsorbed CG surface. Additionally, immunofluorescence studies demonstrated stronger vinculin signals associated with actin stress fibers in the outer regions of the cells and cellular extensions on protein adsorbed NG/UFG surface. The functional response followed the sequence: NG/UFG(BSA) > NG/UFG > CG(BSA) > CG. The differences in the cellular response on bare and protein adsorbed NG/UFG and CG surfaces are attributed to cumulative contribution of grain structure and degree of hydrophilicity. The study underscores the potential advantages of protein adsorption on artificial biomedical devices to enhance the bioactivity and regulate biological functions.
蛋白质的快速吸附是生物医学设备植入生理系统时发生的起始和主要的生物学反应。然而，生物反应取决于设备的表面特性。考虑到对纳米/超细表面和纳米结构涂层的极大兴趣，我们在这里描述了晶粒结构和蛋白质吸附（牛血清白蛋白：BSA）之间的相互作用，通过比较纳米/超细晶粒（NG/UFG）和粗晶粒（CG：晶粒尺寸在微米范围内）来研究细胞-基底相互作用。NG/UFG 表面上的蛋白质吸附有利于调节包括细胞附着、增殖和活力在内的生物功能，而在 CG 表面上吸附蛋白质的效果则不那么明显。此外，免疫荧光研究表明，在 NG/UFG 表面上吸附蛋白质的细胞的外区域与肌动蛋白应力纤维和细胞延伸相关的 vinculin 信号更强。功能反应遵循以下顺序：NG/UFG(BSA) > NG/UFG > CG(BSA) > CG。裸 NG/UFG 和 CG 表面以及吸附蛋白质的 NG/UFG 和 CG 表面上细胞反应的差异归因于晶粒结构和亲水性程度的累积贡献。该研究强调了蛋白质在人工生物医学设备上的吸附在增强生物活性和调节生物功能方面的潜在优势。
