Research Institute of Innovative Technology for the Earth (RITE), 9-2 Kizugawadai, Kizugawa, Kyoto 619-0292, Japan.
Microbiology (Reading). 2010 May;156(Pt 5):1335-1341. doi: 10.1099/mic.0.036913-0. Epub 2010 Jan 28.
Corynebacterium glutamicum cgR_2930 (cyeR) encodes a transcriptional regulator of the ArsR family. Its gene product, CyeR, was shown here to repress the expression of cyeR and the cgR_2931 (cye1)-cgR_2932 operon, which is located upstream of cyeR in the opposite orientation. The cye1 gene encodes an Old Yellow Enzyme family protein, members of which have been implicated in the oxidative stress response. CyeR binds to the intergenic region between cyeR and cye1. Expression of cyeR and cye1 is induced by oxidative stress, and the DNA-binding activity of CyeR is impaired by oxidants such as diamide and H(2)O(2). CyeR contains two cysteine residues, Cys-36 and Cys-43. Whereas mutation of the former (C36A) has no effect on the redox regulation of CyeR activity, mutating the latter (C43A, C43S) abolishes the DNA-binding activity of CyeR. Cys-43 of CyeR and its C36A derivative are modified upon treatment with diamide, suggesting an important role for Cys-43 in the redox regulation of CyeR activity. It is concluded that CyeR is a redox-sensing transcriptional regulator that controls cye1 expression.
谷氨酸棒杆菌 cgR_2930（cyeR）编码 ArsR 家族的转录调节因子。其基因产物 CyeR 被证明可抑制 cyeR 和 cgR_2931（cye1）-cgR_2932 操纵子的表达，该操纵子位于 cyeR 的上游，呈反向排列。cye1 基因编码一个 Old Yellow Enzyme 家族蛋白，该家族成员与氧化应激反应有关。CyeR 结合在 cyeR 和 cye1 之间的基因间区域。氧化应激诱导 cyeR 和 cye1 的表达，并且 CyeR 的 DNA 结合活性被诸如双脒和 H2O2 等氧化剂损害。CyeR 包含两个半胱氨酸残基，Cys-36 和 Cys-43。虽然前者（C36A）的突变对 CyeR 活性的氧化还原调节没有影响，但后者（C43A、C43S）的突变则使 CyeR 的 DNA 结合活性丧失。CyeR 的 Cys-43 和其 C36A 衍生物在双脒处理后发生修饰，表明 Cys-43 在 CyeR 活性的氧化还原调节中起着重要作用。结论是，CyeR 是一种氧化还原感应转录调节因子，可控制 cye1 的表达。
