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Binding site of alpha 2-plasmin inhibitor to plasminogen.α2-纤溶酶抑制剂与纤溶酶原的结合位点。
Biochim Biophys Acta. 1988 Jan 4;952(1):1-7. doi: 10.1016/0167-4838(88)90094-5.
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Substrate kringle-mediated catalysis by the streptokinase-plasmin activator complex: critical contribution of kringle-4 revealed by the mutagenesis approaches.链激酶 - 纤溶酶激活剂复合物的底物kringle介导的催化作用：诱变方法揭示的kringle - 4的关键作用
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Glycolytic and non-glycolytic functions of Mycobacterium tuberculosis fructose-1,6-bisphosphate aldolase, an essential enzyme produced by replicating and non-replicating bacilli.结核分枝杆菌果糖-1,6-二磷酸醛缩酶的糖酵解和非糖酵解功能，该酶是复制和非复制细菌产生的必需酶。
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本文引用的文献

1
X-ray crystal structure of the fibrinolysis inhibitor alpha2-antiplasmin.纤溶酶抑制剂α2-抗纤溶酶的X射线晶体结构。
Blood. 2008 Feb 15;111(4):2049-52. doi: 10.1182/blood-2007-09-114215. Epub 2007 Dec 6.
2
The presence of N-terminal secretion signal sequences leads to strong stimulation of the total expression levels of three tested medically important proteins during high-cell-density cultivations of Escherichia coli.在大肠杆菌的高细胞密度培养过程中，N 端分泌信号序列的存在会强烈刺激三种经测试的医学重要蛋白质的总表达水平。
Appl Environ Microbiol. 2007 Feb;73(3):906-12. doi: 10.1128/AEM.01804-06. Epub 2006 Dec 1.
3
The interaction between plasminogen and antiplasmin variants as studied by surface plasmon resonance.通过表面等离子体共振研究纤溶酶原与抗纤溶酶变体之间的相互作用。
Biochim Biophys Acta. 2006 Nov;1764(11):1730-4. doi: 10.1016/j.bbapap.2006.09.009. Epub 2006 Sep 26.
4
Approaches to selective peptidic inhibitors of factor Xa.凝血因子Xa选择性肽类抑制剂的研究方法。
Chem Biol Drug Des. 2006 Jul;68(1):11-9. doi: 10.1111/j.1747-0285.2006.00404.x.
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The SWISS-MODEL workspace: a web-based environment for protein structure homology modelling.SWISS-MODEL工作区：一个用于蛋白质结构同源建模的基于网络的环境。
Bioinformatics. 2006 Jan 15;22(2):195-201. doi: 10.1093/bioinformatics/bti770. Epub 2005 Nov 13.
6
Functional hierarchy of plasminogen kringles 1 and 4 in fibrinolysis and plasmin-induced cell detachment and apoptosis.纤溶酶原kringle 1和4在纤维蛋白溶解以及纤溶酶诱导的细胞脱离和凋亡中的功能层级
FEBS J. 2005 Jul;272(13):3387-400. doi: 10.1111/j.1742-4658.2005.04754.x.
7
Kringle 5 of human plasminogen induces apoptosis of endothelial and tumor cells through surface-expressed glucose-regulated protein 78.人纤溶酶原的kringle 5通过表面表达的葡萄糖调节蛋白78诱导内皮细胞和肿瘤细胞凋亡。
Cancer Res. 2005 Jun 1;65(11):4663-72. doi: 10.1158/0008-5472.CAN-04-3426.
8
Cold-shock induced high-yield protein production in Escherichia coli.冷休克诱导大肠杆菌高产蛋白质。
Nat Biotechnol. 2004 Jul;22(7):877-82. doi: 10.1038/nbt984. Epub 2004 Jun 13.
9
Inhibition of plasmin-mediated prostromelysin-1 activation by interaction of long chain unsaturated fatty acids with kringle 5.长链不饱和脂肪酸与kringle 5相互作用对纤溶酶介导的前基质金属蛋白酶-1激活的抑制作用。
Biochem Pharmacol. 2004 Feb 15;67(4):643-54. doi: 10.1016/j.bcp.2003.09.033.
10
A novel plasma proteinase potentiates alpha2-antiplasmin inhibition of fibrin digestion.一种新型血浆蛋白酶增强α2-抗纤溶酶对纤维蛋白消化的抑制作用。
Blood. 2004 May 15;103(10):3783-8. doi: 10.1182/blood-2003-12-4240. Epub 2004 Jan 29.

人α(2)-纤溶酶抑制剂：独特的纤溶酶（原）结合区域的功能特征。

The human alpha(2)-plasmin inhibitor: functional characterization of the unique plasmin(ogen)-binding region.

机构信息

Department of Chemistry and Biochemistry, University of Bern, Freiestrasse 3, 3012 Bern, Switzerland.

出版信息

Cell Mol Life Sci. 2010 May;67(9):1505-18. doi: 10.1007/s00018-010-0264-3. Epub 2010 Jan 29.

DOI:10.1007/s00018-010-0264-3
PMID:20112045
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC11115796/
Abstract

The human alpha(2)-plasmin inhibitor (A2PI) possesses unique N- and C-terminal extensions that significantly influence its biological activities. The C-terminal segment, A2PIC (Asn(398)-Lys(452)), contains six lysines thought to be involved in the binding to lysine-binding sites in the kringle domains of human plasminogen, of which four (Lys(422), Lys(429), Lys(436), Lys(452)) are completely and two (Lys(406), Lys(415)) are partially conserved. Multiple Lys to Ala mutants of A2PIC were expressed in Escherichia coli and used in intrinsic fluorescence titrations with kringle domains K1, K4, K4 + 5, and K1 + 2 + 3 of human plasminogen. We were able to identify the C-terminal Lys(452) as the main binding partner in recombinant A2PIC (rA2PIC) constructs with isolated kringles. We could show a cooperative, zipper-like enhancement of the interaction between C-terminal Lys(452) and internal Lys(436) of rA2PIC and isolated K1 + 2 + 3, whereas the other internal lysine residues contribute only to a minor extent to the binding process. Sulfated Tyr(445) in the unique C-terminal segment revealed no influence on the binding affinity to kringle domains.

摘要

人α(2)-纤溶酶抑制剂 (A2PI) 具有独特的 N-末端和 C-末端延伸，这显著影响其生物学活性。C-末端片段 A2PIC（Asn(398)-Lys(452)）含有六个赖氨酸，据认为这些赖氨酸参与与人类纤溶酶原kringle 结构域中的赖氨酸结合位点结合，其中四个（Lys(422)、Lys(429)、Lys(436)、Lys(452)）完全保守，两个（Lys(406)、Lys(415)）部分保守。在大肠杆菌中表达了 A2PIC 的多个 Lys 到 Ala 突变体，并用于与人类纤溶酶原kringle 结构域 K1、K4、K4 + 5 和 K1 + 2 + 3 的固有荧光滴定。我们能够鉴定出 C-末端 Lys(452)是与分离的 kringles 结合的重组 A2PIC(rA2PIC)构建体中的主要结合伴侣。我们可以显示出 C-末端 Lys(452)与 rA2PIC 和分离的 K1 + 2 + 3 之间的拉链式协同增强相互作用，而其他内部赖氨酸残基仅对结合过程有较小的贡献。独特的 C-末端片段中的硫酸化 Tyr(445)对与 kringle 结构域的结合亲和力没有影响。