Kysela B, Matoska J
Cancer Research Institute, Slovak Academy of Sciences, Bratislava.
Neoplasma. 1991;38(1):3-11.
In a series of 49 cases of seminomas, namely 19 classical seminomas, 21 seminomas with syncytiotrophoblastic cells and 9 spermatocytic seminomas, DNA ploidy and S-phase cell fraction of the cell cycle were estimated in paraffin-embedded histopathological material. DNA aneuploidy was detected in 16/19 classical seminomas (84%), in all seminomas with syncytiotrophoblastic cells (100%) and in 6/9 spermatocytic seminomas (67%). In three cases two distinct aneuploid stemlines were detected, in four cases regional variations in ploidy level were observed, clearly proving cellular heterogeneity within the studied specimens. No significant differences in distribution of ploidy levels of aneuploid tumors were detected either between distinct groups of seminomas or in relation to the age of the patients. On the other hand, mean values of S-phase cell fractions in our material offer statistically highly significant differences between defined groups of tumors. Spermatocytic seminomas had the highest level of proliferation activity, which is in contrast with the clinicopathological observations (relatively slow growth, rare occurrence of metastases, local malignancy). The results of proliferation activity analysis and the relatively highest incidence of diploid tumors support the theory of different origin of spermatocytic seminomas in comparison with other germ cell tumors.
在一组49例精原细胞瘤中，即19例经典型精原细胞瘤、21例伴有合体滋养层细胞的精原细胞瘤和9例精母细胞性精原细胞瘤，对石蜡包埋的组织病理学材料进行细胞周期的DNA倍体和S期细胞分数测定。在19例经典型精原细胞瘤中有16例（84%）检测到DNA非整倍体，所有伴有合体滋养层细胞的精原细胞瘤（100%）以及9例精母细胞性精原细胞瘤中的6例（67%）检测到DNA非整倍体。在3例中检测到两种不同的非整倍体干系，在4例中观察到倍体水平的区域差异，清楚地证明了所研究标本内的细胞异质性。在不同组的精原细胞瘤之间或与患者年龄相关的非整倍体肿瘤倍体水平分布上未检测到显著差异。另一方面，我们材料中S期细胞分数的平均值在特定肿瘤组之间具有统计学上的高度显著差异。精母细胞性精原细胞瘤具有最高水平的增殖活性，这与临床病理观察结果（生长相对缓慢、转移罕见、局部恶性）相反。增殖活性分析结果和二倍体肿瘤的相对最高发生率支持精母细胞性精原细胞瘤与其他生殖细胞肿瘤起源不同的理论。
