纳米凝胶自组装包埋脂肪酶：高酶活诱导及热稳定性增强。

Nano-encapsulation of lipase by self-assembled nanogels: induction of high enzyme activity and thermal stabilization.

机构信息

Institute of Biomaterials and Bioengineering, Tokyo Medical and Dental University, 2-3-10 Kanda-surugadai, Chiyoda-ku, Tokyo 101-0062, Japan.

出版信息

Macromol Biosci. 2010 Apr 8;10(4):353-8. doi: 10.1002/mabi.200900304.

DOI:10.1002/mabi.200900304

PMID:20112238

Abstract

The effects of self-assembled polysaccharide nanogels on colloidal and thermal stability of lipase from Pseudomonas cepacia were investigated. The enzyme activity, especially k(cat), drastically increased in the presence of nanogels of cholesterol-bearing pullulan (CHP). The thermostability of lipase complex increased because the denaturation temperature of lipase increased by more than 20 degrees C by complexation with CHP nanogels. Lipase denaturation and aggregation upon heating was effectively prevented by complexation with CHP nanogels. Moreover, complexation with CHP nanogels protected lipase from lyophilization-induced aggregation. Nano-encapsulation with CHP nanogel is a useful method for colloidal and thermal stabilization of unstable enzyme.

摘要

研究了自组装多糖纳米凝胶对假单胞菌脂肪酶胶体和热稳定性的影响。在存在带胆固醇的普鲁兰（CHP）纳米凝胶的情况下，酶活性，特别是 k(cat)，急剧增加。脂肪酶与 CHP 纳米凝胶形成复合物后，其热稳定性增加，因为脂肪酶的变性温度升高了 20 多摄氏度。通过与 CHP 纳米凝胶的复合，可以有效地防止脂肪酶在加热时变性和聚集。此外，与 CHP 纳米凝胶的复合还可以防止脂肪酶在冻干过程中聚集。用 CHP 纳米凝胶进行纳米封装是一种稳定不稳定酶的胶体和热稳定性的有用方法。