Wu Qi, Shan Zhi, Shen Mao, Li Shuangjiang, Chen Hui
College of Life Science, Sichuan Agricultural University, Ya'an 625014, China.
Sheng Wu Gong Cheng Xue Bao. 2009 Oct;25(10):1477-82.
We prepared the nano-sized magnetic fluid in aqueous phase solution to modify the killed yeast cells (Saccharomyces cerevisiae). Subsequently, the yeast cells with good magnetic response was obtained.The infrared spectroscopy analysis showed that the modified yeast cells increased obviously the absorption at the Fe-O characteristic peak of 581 cm(-1). Moreover, the transmission electron microscopy picture indicated the magnetic nanoparticles presented individually or aggregately on the yeast cell surface. Under the experimental conditions, the magnetically labeled yeast with 160 microL showed the adsorption percentage up to 100% in 1 mL direct scarlet dye solution with the concentration of 0.4 mg/mL, and arrived at the adsorption equilibrium within 8 min. Besides, the adsorbed dyes can be eluted in 70% alcohol with a desorption percentage of 99.18%. Because of the strong adsorption capacity, the high adsorption speed and easy of magnetic separation, the magnetically modified yeast cells are the promising biosorbent candidates for removal of water-soluble dyes.
我们在水相溶液中制备了纳米级磁性流体以修饰灭活的酵母细胞(酿酒酵母)。随后,获得了具有良好磁响应的酵母细胞。红外光谱分析表明,修饰后的酵母细胞在581 cm(-1)的Fe-O特征峰处的吸收明显增加。此外,透射电子显微镜图像显示磁性纳米颗粒单独或聚集在酵母细胞表面。在实验条件下,160 μL的磁性标记酵母在浓度为0.4 mg/mL的1 mL直接猩红染料溶液中的吸附率高达100%,并在8分钟内达到吸附平衡。此外,吸附的染料可以用70%的酒精洗脱,解吸率为99.18%。由于具有较强的吸附能力、较高的吸附速度和易于磁分离的特点,磁性修饰的酵母细胞是去除水溶性染料的有前途的生物吸附剂候选物。