[Transport and metabolism of daidzein in Caco-2 cell model].

OBJECTIVE

To investigate the intestinal absorption and metabolism of daidzein in Caco-2 cell model.

METHODS

The damage of daidzein to Caco-2 cell was evaluated by MTT value and alkaline phosphatase (ALP) and lactate dehydrogenase (LDH) activity. With the reference to propranolol, the apparent permeability coefficient (Papp) of daidzein was measured through the monolayer under the different concentrations and pH media. The metabolites were also measured by enzyme hydrolysis.

RESULTS

Daidzein had no damage to the growth of the Caco-2 cells in the concentration of 1-50 microg/mL. The apparent permeability coefficient (Papp) of daidzein across the monolayer showed concentration- and pH- independent manner, which was similar to that of transcellcular marker-propranolol, suggesting the good absorption of daidzein in vivo. By hydrolysis with beta-glucuronidase, low metabolites were detected in monolayer and transport medium, verifying the existence of glucuronides and sulfates.

CONCLUSION

The daidzein absorption in Caco-2 cell model is a passive and transcellular transport and quite good in the intestines.