Ge Yue-Bin, Ma Yan, Li Wei-Zhong
Department of Pharmaceutics, School of Life Sciences, South-Central University for Nationalities, Wuhan 430074, China.
Zhong Yao Cai. 2009 Oct;32(10):1563-7.
To investigate the intestinal absorption and metabolism of daidzein in Caco-2 cell model.
The damage of daidzein to Caco-2 cell was evaluated by MTT value and alkaline phosphatase (ALP) and lactate dehydrogenase (LDH) activity. With the reference to propranolol, the apparent permeability coefficient (Papp) of daidzein was measured through the monolayer under the different concentrations and pH media. The metabolites were also measured by enzyme hydrolysis.
Daidzein had no damage to the growth of the Caco-2 cells in the concentration of 1-50 microg/mL. The apparent permeability coefficient (Papp) of daidzein across the monolayer showed concentration- and pH- independent manner, which was similar to that of transcellcular marker-propranolol, suggesting the good absorption of daidzein in vivo. By hydrolysis with beta-glucuronidase, low metabolites were detected in monolayer and transport medium, verifying the existence of glucuronides and sulfates.
The daidzein absorption in Caco-2 cell model is a passive and transcellular transport and quite good in the intestines.
在Caco-2细胞模型中研究大豆苷元的肠道吸收及代谢情况。
通过MTT值、碱性磷酸酶（ALP）及乳酸脱氢酶（LDH）活性评估大豆苷元对Caco-2细胞的损伤。以普萘洛尔为参照，在不同浓度及pH介质条件下，测定大豆苷元透过单层细胞的表观渗透系数（Papp）。同时通过酶水解法测定代谢产物。
在1 - 50μg/mL浓度范围内，大豆苷元对Caco-2细胞生长无损伤。大豆苷元透过单层细胞的表观渗透系数（Papp）呈现出与浓度及pH无关的特性，这与跨细胞标志物普萘洛尔相似，表明大豆苷元在体内具有良好的吸收性。经β-葡萄糖醛酸酶水解后，在单层细胞及转运介质中检测到少量代谢产物，证实了葡萄糖醛酸苷和硫酸盐的存在。
Caco-2细胞模型中大豆苷元的吸收是一种被动的跨细胞转运过程，且在肠道中吸收良好。
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