[葛根素及其纳米粒经Caco-2细胞模型的吸收机制研究]
[Study on absorption mechanism of puerarin and its nanoparticles across Caco-2 cell model].
作者信息
Liu Xi-Jing, Jia Qiang, Wang Cheng-Yuan, Wang Nai-Li
机构信息
Research Institute of Qinghua University in Shenzhen, Shenzhen 518057, China.
出版信息
Zhong Yao Cai. 2009 Aug;32(8):1252-5.
OBJECTIVE
To study the absorption mechanism of puerarin and its nanoparticles across Caco-2 cell model.
METHODS
The Caco-2 monolayer model was used to explore the effect of time and drug concentration on the absorption of puerarin and its nanoparticeles. The drug concentration was determined by high-performance liquid chromatography and the Papp was caculated.
RESULTS
The puerarin-PLGA-NP could significantly improve the absorption of puerarin. Puerarin was not only absorbed simply through passive diffusion, but also had relations with high dispersion of nanoparticles, increase of affinity and interaction with intestinal mucous membrane.
CONCLUSION
The main mechanism of puerarin and its nanoparticles across Caco-2 monolayer model is passive transference. Puerarin-NP could increase the absorption of the drug in intestinal epithlial.
目的
研究葛根素及其纳米粒通过Caco-2细胞模型的吸收机制。
方法
采用Caco-2单层细胞模型探讨时间和药物浓度对葛根素及其纳米粒吸收的影响。通过高效液相色谱法测定药物浓度并计算表观渗透系数（Papp）。
结果
葛根素-聚乳酸-羟基乙酸共聚物纳米粒（puerarin-PLGA-NP）可显著提高葛根素的吸收。葛根素不仅通过被动扩散简单吸收，还与纳米粒的高分散性、亲和力增加以及与肠黏膜的相互作用有关。
结论
葛根素及其纳米粒通过Caco-2单层细胞模型的主要机制是被动转运。葛根素纳米粒可增加药物在肠上皮的吸收。
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