穿心莲内酯和脱水穿心莲内酯表观油水分配系数的测定及其pH影响
[Determination of apparent oil-water partition coefficient of andrographolide and dehydroandrographolide and effect of pH on them].
作者信息
Yao Yuan, Liao Qiong-Feng, Zeng Li-Ying, Zhang Lei, Ma Yan, Zeng Yuan-Er
机构信息
College of Chinese Traditional Medicine, Guangzhou University of Chinese Medicine, Guangzhou 510006, China.
出版信息
Zhong Yao Cai. 2009 Oct;32(10):1610-2.
OBJECTIVE
To determine the apparent n-octanol-water/buffer partition coefficient of andrographolide and dehydroandrographolide.
METHODS
The apparent n-octanol-water/buffer partition coefficients of andrographolide and dehydroandrographolide were measured by shaking flask method. The concentrations of andrographolide and dehydroandrographolide were analyzed by HPLC method.
RESULTS
The Papp of andrographolide and dehydroandrographolide were 3.90 (log Papp = 0.59) and 19.75 (log Papp = 1. 30) in water at 37 degrees C, respectively.
CONCLUSIONS
The apparent n-octanol-buffer partition coefficients of andrographolide and dehydroandrographolide are influenced by pH, and the higher pH may decrease the apparent n-octanol-buffer partition coefficients of them. Andrographolide has the highest partition coefficient in pH6, and dehydroandrogapholide in pH5.
目的
测定穿心莲内酯和脱水穿心莲内酯的正辛醇 - 水/缓冲液表观分配系数。
方法
采用摇瓶法测定穿心莲内酯和脱水穿心莲内酯的正辛醇 - 水/缓冲液表观分配系数。通过高效液相色谱法分析穿心莲内酯和脱水穿心莲内酯的浓度。
结果
在37℃水中，穿心莲内酯和脱水穿心莲内酯的Papp分别为3.90（log Papp = 0.59）和19.75（log Papp = 1.30）。
结论
穿心莲内酯和脱水穿心莲内酯的正辛醇 - 缓冲液表观分配系数受pH影响，较高的pH可能会降低它们的正辛醇 - 缓冲液表观分配系数。穿心莲内酯在pH6时分配系数最高，脱水穿心莲内酯在pH5时分配系数最高。
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