State Key Laboratory of Pulp and Paper Engineering, South China University of Technology, Guangzhou 510641, PR China.
J Agric Food Chem. 2010 Feb 24;58(4):2253-9. doi: 10.1021/jf903731s.
The process of conversion of bamboo fiber into fermentable glucose in the formic acid reaction system was investigated using cross-polarization/magic angle spinning (13)C-nuclear magnetic resonance (CP/MAS (13)C NMR), X-ray diffraction. The results indicated that formic acid as an active agent was able to effectively penetrate into the interior space of the cellulose molecules, thus collapsing the rigid crystalline structure and allowing hydrolysis to occur easily in the amorphous zone as well as in the crystalline zone. The bamboo fiber was hydrolyzed using formic acid and 4% hydrochloric acid under mild conditions. The effects of temperature (55-75 degrees C), retention time (0-9 h) and catalyst, the concentration of glucose and the degradation pathway of glucose were analyzed. The main degradation pathway of glucose is that the hydroxyl group on the 2-carbon is protonated and cleaved off. Aluminum iso-propoxide prevented the degradation of glucose, and the acetone promoted the degradation of glucose to levulinic acid.
采用交叉极化/魔角旋转（13）C 核磁共振（CP/MAS（13）C NMR）、X 射线衍射研究了在甲酸反应体系中，竹纤维转化为可发酵葡萄糖的过程。结果表明，甲酸作为一种活性试剂能够有效地渗透到纤维素分子的内部空间，从而使刚性结晶结构坍塌，使无定形区和结晶区易于水解。在温和条件下，使用甲酸和 4%盐酸水解竹纤维。分析了温度（55-75℃）、保温时间（0-9 h）、催化剂、葡萄糖浓度和葡萄糖的降解途径的影响。葡萄糖的主要降解途径是 2-碳上的羟基质子化并断裂。异丙醇铝阻止了葡萄糖的降解，丙酮促进了葡萄糖向乙酰丙酸的降解。
