Dujovne Irene, Kerssemakers Jacob, Cappello G, Dekker Cees
Kavli Institute of NanoScience, Delft University of Technology, Lorentzweg 1, 2628 CJ Delft, The Netherlands.
Rev Sci Instrum. 2010 Jan;81(1):016103. doi: 10.1063/1.3280167.
We present a novel high-resolution technique for single-molecule experiments, viz., differential traveling wave tracking. This is an interference-based scattering technique where we use gold nanoparticles for high scattering intensities and incorporate differential measurements along one in-plane direction to subtract mechanical noise and drift of the system. In addition, out-of-plane distances are measured via scattered light intensity in a total internal reflectance illumination field. In plane, we demonstrate a rms noise level of only 0.10 nm at 10 kHz and less than 0.5 nm at 600 kHz.
我们提出了一种用于单分子实验的新型高分辨率技术，即差分行波跟踪。这是一种基于干涉的散射技术，我们使用金纳米颗粒以获得高散射强度，并沿一个平面内方向进行差分测量，以减去系统的机械噪声和漂移。此外，通过全内反射照明场中的散射光强度来测量平面外距离。在平面内，我们证明在10 kHz时均方根噪声水平仅为0.10 nm，在600 kHz时小于0.5 nm。
