利用结构光照明显微术对金纳米粒子的亚衍射散射光成像。

Subdiffraction scattered light imaging of gold nanoparticles using structured illumination.

机构信息

National Synchrotron Radiation Research Center, Hsinchu Science Park, Hsinchu, Taiwan.

出版信息

Opt Lett. 2011 Dec 15;36(24):4773-5. doi: 10.1364/OL.36.004773.

DOI:10.1364/OL.36.004773

Abstract

A reflective light-scattering (RLS) microscope with structured illumination (SI) provides subdiffraction resolution and improves the image quality of gold nanoparticles in biological systems. The three-dimensional (3D)-structured pattern is rapidly and precisely controlled with a spatial light modulator and scrambled at the conjugate image plane to increase spatial incoherence. The reconstructed SI-RLS image of 100 nm gold nanoparticles reveals lateral and axial resolutions of approximately 117 and 428 nm. We present a high-resolution image of gold nanoparticles inside a HeLa cell, with improved contrast.

摘要

反射光散射（RLS）显微镜结合结构光照（SI）技术提供了亚衍射分辨率，并提高了生物体系中金纳米粒子的图像质量。通过空间光调制器可以快速、精确地控制三维（3D）结构图案，并在共轭像面进行混频以增加空间非相干性。对 100nm 金纳米粒子的重构 SI-RLS 图像显示，其横向和轴向分辨率约为 117nm 和 428nm。我们呈现了金纳米粒子在 HeLa 细胞内的高分辨率图像，对比度得到了改善。