Program in Public Health, University of California, Irvine, CA 92697-3900, USA.
Insect Mol Biol. 2010 Apr;19(2):263-9. doi: 10.1111/j.1365-2583.2009.00986.x. Epub 2010 Jan 24.
Genetically modified mosquitoes that are unable to transmit pathogens offer opportunities for controlling vector-borne diseases such as malaria and dengue. Site-specific gene recombination technologies are advantageous in the development of these insects because antipathogen effector genes can be inserted at integration sites in the genome that cause the least alteration in mosquito fitness. Here we describe Anopheles stephensi transgenic lines containing phi C31 attP'docking' sites linked to a fluorescent marker gene. Chromosomal insertion sites were determined and life-table parameters were assessed for transgenic mosquitoes of each line. No significant differences in fitness between the transgenic and nontransgenic mosquitoes were detected in this study. These transgenic lines are suitable for future site-specific integrations of antiparasite transgenes into the attP sites.
无法传播病原体的转基因蚊子为控制疟疾和登革热等媒介传播疾病提供了机会。 基因定点重组技术在这些昆虫的发展中具有优势，因为抗病原体效应基因可以插入基因组中的整合位点，从而对蚊子的适应性造成最小的改变。 在这里，我们描述了含有 phi C31 attP“对接”位点的转基因 Anopheles stephensi 品系，该位点与荧光标记基因相连。 确定了染色体插入位点，并评估了每条品系转基因蚊子的生命表参数。 在这项研究中，未检测到转基因蚊子与非转基因蚊子在适应性方面有显着差异。 这些转基因品系适合将来将寄生虫转基因进行基因定点整合到 attP 位点。
