Department of Environmental Science (Chemistry), Sir Wilfred Grenfell College, Memorial University of Newfoundland, Corner Brook, NL, Canada A2H 6P9.
Anal Chim Acta. 2010 Feb 28;661(2):181-7. doi: 10.1016/j.aca.2009.12.027. Epub 2009 Dec 24.
The estimate of ergosterol ([5,7,22-ergostatrien-3beta-ol] has been used by many to relate its concentration to the amount of mold in soils. This new method using on-fiber derivatization-solid phase microextraction-GC/MS method for the analysis of ergosterol presents a quick and straightforward method where low detection limits (1.5 ppb) and good limit of quantitation range (3 ppb to 90 ppm) can be achieved with careful control of analytical parameters. After saponification of real soil samples, sampling without extensive workup can be performed and analysis by a standard addition method can be utilized to deduce the original sample concentration of ergosterol. Peak area extraction analysis by MS SIM on selected characteristic fragment ions gives results with RSD < or = 2.2%.
麦角甾醇（[5,7,22-麦角甾三烯-3β-醇]的浓度已被许多人用来估算土壤中霉菌的数量。这种新的方法使用纤维内衍生化-固相微萃取-GC/MS 法来分析麦角甾醇，提供了一种快速而直接的方法，通过仔细控制分析参数，可以实现低检测限（1.5 ppb）和良好的定量范围限（3 ppb 至 90 ppm）。对实际土壤样品进行皂化后，无需进行广泛的处理即可进行采样，并可以通过标准添加法进行分析，以推断麦角甾醇原始样品的浓度。通过对选定的特征碎片离子进行 MS SIM 峰面积提取分析，结果的 RSD < 或 = 2.2%。
