Liu Guoxing, Xie Chengzhi, Sun Fadi, Xu Xundi, Yang Yaohui, Zhang Tao, Deng Yuansheng, Wang Dianchen, Huang Zhao, Yang Leping, Huang Shengfu, Wang Qunwei, Liu Guoli, Zhong Dewu, Miao Xiongying
Department of Surgery, The 2nd Xiangya Hospital, Central South University, Renmin Zhong Road 139, Changsha City, Hunan Province, China.
Cancer Genet Cytogenet. 2010 Feb;197(1):54-9. doi: 10.1016/j.cancergencyto.2009.08.007.
Transient receptor potential vanilloid 2 (TRPV2), one of the members of TRP (transient receptor potential) superfamily of ion channels, has been suggested to contribute to pain associated with inflammation or neuropathy. To investigate its role in hepatocarcinogenesis, we examined the expression of TRPV2 in human hepatocellular carcinoma (HCC) samples and analyzed the association of TRPV2 expression with its clinical significance. TRPV2 expression in 55 HCC patients was examined by immunohistochemistry, and the correlation between TRPV2 levels and clinicopathologic parameters was analyzed. Thirteen paired HCC specimens and their nontumor counterparts were investigated by quantitative real-time polymerase chain reaction (RT-PCR) and Western blotting, respectively. Quantitative RT-PCR and Western blotting analysis revealed that expression of TRPV2 at both the mRNA and protein levels were increased in cirrhotic livers compared with chronic hepatitis, whereas that also occurred in moderately and well-differentiated tumors compared with that of poorly differentiated tumors. Immunohistochemistry of the 55 HCC samples showed that the expression of TRPV2 increased when going from normal liver or chronic hepatitis to cirrhosis. Increased TRPV2 expression was observed in tissues of liver cirrhosis (31/37, 83.8%). In HCC, increased expression of TRPV2 was identified in 16/55 (29%) cases. Clinicopathologic assessment suggested a significant association between TRPV2 expression and portal vein invasion and histopathologic differentiation (P = 0.036 and 0.001, respectively). Our data suggest that TRPV2 plays a role in human hepatocarcinogenesis and might be a prognostic marker of patients with HCC.
瞬时受体电位香草酸亚型2（TRPV2）是瞬时受体电位（TRP）离子通道超家族的成员之一，有人认为它与炎症或神经病变相关的疼痛有关。为了研究其在肝癌发生中的作用，我们检测了人肝细胞癌（HCC）样本中TRPV2的表达，并分析了TRPV2表达与其临床意义之间的关联。通过免疫组织化学检测了55例HCC患者中TRPV2的表达，并分析了TRPV2水平与临床病理参数之间的相关性。分别通过定量实时聚合酶链反应（RT-PCR）和蛋白质印迹法对13对HCC标本及其癌旁非肿瘤组织进行了研究。定量RT-PCR和蛋白质印迹分析显示，与慢性肝炎相比，肝硬化肝脏中TRPV2在mRNA和蛋白质水平的表达均增加，而与低分化肿瘤相比，中高分化肿瘤中也出现这种情况。对55例HCC样本进行的免疫组织化学检测显示，从正常肝脏或慢性肝炎发展到肝硬化时，TRPV2的表达增加。在肝硬化组织中观察到TRPV2表达增加（31/37，83.8%）。在HCC中，16/55（29%）例中发现TRPV2表达增加。临床病理评估表明，TRPV2表达与门静脉侵犯和组织病理学分化之间存在显著关联（分别为P = 0.036和0.001）。我们的数据表明，TRPV2在人类肝癌发生中起作用，可能是HCC患者的一个预后标志物。
