Department of Neurobiology Institute for Biological Research, University of Belgrade, Bulevar despota Stefana 142, 11060 Belgrade, Serbia.
Brain Res. 2010 Mar 19;1321:96-104. doi: 10.1016/j.brainres.2010.01.048. Epub 2010 Jan 28.
The changes that occur during adolescence have a profound impact on the brain and behavior later in life. In this work we examined changes in motor activity during habituation to a novel environment and after treatment with MK-801 (0.025, 0.05, 0.1mg/kg) in peripubertal, pubertal and adult Wistar rats. The involvement of the motor cortex and striatum in motor activity was assessed by analyzing changes in c-Fos protein levels that served as an indicator of neuronal activity. During the habituation period, locomotor activity in peripubertal rats was higher during the first 10 min than in other groups. The same amount of stereotypy-like movements was detected in all three groups. MK-801 induced dose- and age-dependent changes in motor activity. Peripubertal rats were the most sensitive to treatment with MK-801. We also report a surprising finding that systemic application of MK-801 induced a similar age-related profile of changes in motor activity and c-Fos protein expression in the motor cortex but no c-Fos induction in the striatum. Our results demonstrate that, depending on the phase of adolescence the same dose of MK-801 affected behavioral functions in a different manner and that activity of the motor cortex rather than striatal activity was linked to drug-motor activity interactions.
青春期发生的变化对大脑和后期的行为有深远影响。在这项工作中，我们研究了在新环境习惯化过程中和在未成年、青春期和成年 Wistar 大鼠中用 MK-801（0.025、0.05、0.1mg/kg）治疗后运动活动的变化。通过分析作为神经元活动指标的 c-Fos 蛋白水平的变化，评估运动皮层和纹状体在运动活动中的参与。在习惯化期间，未成年大鼠在最初 10 分钟内的运动活性高于其他组。在所有三组中都检测到相同数量的刻板样运动。MK-801 诱导运动活性的剂量和年龄依赖性变化。未成年大鼠对 MK-801 的治疗最敏感。我们还报告了一个令人惊讶的发现，即全身应用 MK-801 在运动皮层中诱导出类似的与年龄相关的运动活性和 c-Fos 蛋白表达变化的模式，但在纹状体中没有 c-Fos 诱导。我们的结果表明，取决于青春期的阶段，相同剂量的 MK-801 以不同的方式影响行为功能，并且运动皮层的活性而不是纹状体的活性与药物-运动活性相互作用有关。
