Huh N E, Hwang I W, Lim K, You K H, Chae C B
Department of Biochemistry and Biophysics, University of North Carolina, Chapel Hill 27599-7260.
Nucleic Acids Res. 1991 Jan 11;19(1):93-8. doi: 10.1093/nar/19.1.93.
During mammalian spermatogenesis, somatic histones are replaced by testis-specific variants. The synthesis of the variants occurs primarily in the germ cells undergoing meiosis in the absence of DNA replication. We have cloned the genes encoding rat somatic and testis-specific H2A (TH2A) histones. The two genes share 300 bp of 5' upstream region with respective H2B genes: somatic H2A with somatic H2B and testis-specific TH2A with testis-specific TH2B gene. The deduced amino acid sequences show that H2A and TH2A histones have eight amino acid differences in the first half of the molecules and three consecutive changes in the C-terminal region. TH2A gene is expressed only in testis. Although synthesis of TH2A and TH2B histones is independent of DNA replication and insensitive to inhibitors of DNA synthesis in testis, the regulatory region shared by the two genes contain a bi-directional S phase-specific transcription regulatory element. In addition, TH2A gene, like TH2B gene, contains the consensus sequence element in the 3' non-coding region which is involved in the S phase-specific stabilization of histone mRNA.
在哺乳动物精子发生过程中，体细胞组蛋白被睾丸特异性变体所取代。这些变体的合成主要发生在减数分裂过程中的生殖细胞中，且不存在DNA复制。我们已经克隆了编码大鼠体细胞和睾丸特异性H2A（TH2A）组蛋白的基因。这两个基因与各自的H2B基因共享300 bp的5'上游区域：体细胞H2A与体细胞H2B，睾丸特异性TH2A与睾丸特异性TH2B基因。推导的氨基酸序列表明，H2A和TH2A组蛋白在分子的前半部分有八个氨基酸差异，在C末端区域有三个连续变化。TH2A基因仅在睾丸中表达。尽管TH2A和TH2B组蛋白的合成独立于DNA复制且对睾丸中DNA合成抑制剂不敏感，但这两个基因共享的调控区域包含一个双向S期特异性转录调控元件。此外，TH2A基因与TH2B基因一样，在3'非编码区域含有共有序列元件，该元件参与组蛋白mRNA的S期特异性稳定。
Zotero 插件
