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小鼠骨髓瘤细胞中组蛋白基因转录速率和组蛋白mRNA水平的快速可逆变化。

Rapid reversible changes in the rate of histone gene transcription and histone mRNA levels in mouse myeloma cells.

作者信息

Graves R A, Marzluff W F

出版信息

Mol Cell Biol. 1984 Feb;4(2):351-7. doi: 10.1128/mcb.4.2.351-357.1984.

DOI:10.1128/mcb.4.2.351-357.1984
PMID:6700595
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC368702/
Abstract

The levels of histone mRNAs are reduced 90 to 95% after treatment of mouse myeloma cells with inhibitors of DNA synthesis which disrupt deoxynucleotide metabolism. In contrast, novobiocin, which inhibits DNA synthesis but does not alter deoxynucleotide metabolism, did not alter histone mRNA levels. Upon reversing the inhibition by fluorodeoxyuridine by feeding with thymidine, histone mRNA levels are restored to control levels within 40 to 60 min. The rate of histone gene transcription is reduced 75 to 80% within 10 min after treatment with fluorodeoxyuridine and increased to control levels within 10 min after refeeding with thymidine. Inhibition of protein synthesis with cycloheximide or puromycin in cells which had been treated with fluorodeoxyuridine resulted in an increase of histone mRNA levels. This was partly due to an increase in the rate of transcription. The data indicate that both transcription and mRNA degradation are linked to deoxynucleotide metabolism. Continued protein synthesis is necessary for maintaining the inhibition of histone gene transcription.

摘要

用破坏脱氧核苷酸代谢的DNA合成抑制剂处理小鼠骨髓瘤细胞后，组蛋白mRNA水平降低90%至95%。相比之下，抑制DNA合成但不改变脱氧核苷酸代谢的新生霉素，并未改变组蛋白mRNA水平。在用胸苷补充喂养以逆转氟脱氧尿苷的抑制作用后，组蛋白mRNA水平在40至60分钟内恢复到对照水平。用氟脱氧尿苷处理后10分钟内，组蛋白基因转录速率降低75%至80%，在用胸苷重新补充喂养后10分钟内增加到对照水平。在用氟脱氧尿苷处理过的细胞中，用环己酰亚胺或嘌呤霉素抑制蛋白质合成导致组蛋白mRNA水平增加。这部分是由于转录速率增加。数据表明，转录和mRNA降解均与脱氧核苷酸代谢相关。持续的蛋白质合成对于维持组蛋白基因转录的抑制是必要的。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/c2c3/368702/c3f8bd12305a/molcellb00144-0143-b.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/c2c3/368702/32deac40d937/molcellb00144-0141-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/c2c3/368702/89e72930dcca/molcellb00144-0142-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/c2c3/368702/d609c92950c2/molcellb00144-0143-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/c2c3/368702/c3f8bd12305a/molcellb00144-0143-b.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/c2c3/368702/32deac40d937/molcellb00144-0141-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/c2c3/368702/89e72930dcca/molcellb00144-0142-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/c2c3/368702/d609c92950c2/molcellb00144-0143-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/c2c3/368702/c3f8bd12305a/molcellb00144-0143-b.jpg

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