Institute of Loess Plateau, Shanxi University, Taiyuan, Shanxi 030006, China.
Ecotoxicol Environ Saf. 2010 Jul;73(5):944-8. doi: 10.1016/j.ecoenv.2009.12.026. Epub 2010 Jan 29.
The coking wastewater induces severe environmental problems in China, however, its toxicity has not been well known. In the present study, the genotoxicity of coking wastewater was studied using Vicia faba and Hordeum vulgare root tip cytogenetic bioassays. Results show that the tested coking wastewater decreased the mitotic index, and significantly enhanced the frequencies of micronucleus, sister chromatid exchange and pycnotic cell in concentration-dependent manners. Exposure to the same concentration wastewater, the increasing ratios of above genetic injuries were higher in V. faba than that in H. vulgare. The results imply that coking wastewater is a genotoxic agent in plant cells and exposure to the wastewater in environment may pose a potential genotoxic risk to organisms. It also suggests that both bioassays can be used for testing the genotoxicity of coking wastewater, but the V. faba assay is more sensitive than H. vulgare assay during the process.
焦化废水在中国引发了严重的环境问题，但它的毒性尚未被充分了解。在本研究中，利用蚕豆和大麦根尖细胞遗传生物检测法研究了焦化废水的遗传毒性。结果表明，所测试的焦化废水降低了有丝分裂指数，并以浓度依赖的方式显著增加了微核、姐妹染色单体交换和染色质固缩细胞的频率。在暴露于相同浓度废水的情况下，蚕豆中上述遗传损伤的增加比例高于大麦。结果表明，焦化废水是植物细胞中的遗传毒性物质，在环境中接触该废水可能会对生物造成潜在的遗传毒性风险。这也表明，两种生物测定法都可用于测试焦化废水的遗传毒性，但在这个过程中，蚕豆测定法比大麦测定法更敏感。
