Department of Psychiatry, Institute of Psychiatric Research, Indiana University-Purdue University at Indianapolis, Indianapolis, 46202-4887, USA.
Alcohol. 2010 Mar;44(2):171-83. doi: 10.1016/j.alcohol.2009.12.001. Epub 2010 Jan 29.
The objective of this study was to determine time-course changes in gene expression within two regions of the extended amygdala after binge-like alcohol drinking by alcohol-preferring (P) rats. Adult male P rats were given 1-h access to 15 and 30% ethanol three times daily for 8 weeks. Rats (n = 10/time point for ethanol and n = 6/time point for water) were killed by decapitation 1, 6, and 24 h after the last drinking episode. RNA was prepared from individual micropunch samples of the nucleus accumbens shell (ACB-shell) and central nucleus of the amygdala (CeA); analyses were conducted with Affymetrix Rat Genome 230.2 GeneChips. Ethanol intakes were 1.5-2 g/kg for each of the three sessions. There were no genes that were statistically different between the ethanol and water control groups at any individual time point. Therefore, an overall effect, comparing the water control and ethanol groups, was determined. In the ACB-shell and CeA, there were 276 and 402 probe sets for named genes, respectively, that differed between the two groups. There were 1.5-3.6-fold more genes with increased expression than with decreased expression in the ethanol-drinking group, with most differences between 1.1- and 1.2-fold. Among the differences between the ethanol and water control groups were several significant biological processes categories that were in common between the two regions (e.g., synaptic transmission, neurite development); however, within these categories, there were few genes in common between the two regions. Overall, the results indicate that binge-like alcohol drinking by P rats produces region-dependent changes in the expression of genes that could alter transcription, synaptic function, and neuronal plasticity in the ACB-shell and CeA; within each region, different mechanisms may underlie these alterations because there were few common ethanol-responsive genes between the ACB-shell and CeA.
这项研究的目的是确定在酒精偏好（P）大鼠进行 binge 样饮酒后，两个扩展杏仁核区域的基因表达随时间的变化。成年雄性 P 大鼠每天有 1 小时的时间接触 15%和 30%的乙醇，共 8 周。大鼠（每组 10 只，用于乙醇组；每组 6 只，用于水组）在最后一次饮酒后 1、6 和 24 小时断头处死。从伏隔核壳（ACB-shell）和杏仁核中央核（CeA）的单个微穿孔样本中提取 RNA；使用 Affymetrix Rat Genome 230.2 GeneChips 进行分析。每个 3 个疗程的乙醇摄入量为 1.5-2 g/kg。在任何单个时间点，乙醇组和水对照组之间都没有统计学上差异的基因。因此，确定了水对照组和乙醇组之间的总体效果。在 ACB-shell 和 CeA 中，分别有 276 和 402 个探针组用于命名基因，两组之间存在差异。在乙醇组中，表达增加的基因比表达减少的基因多 1.5-3.6 倍，大多数差异在 1.1-1.2 倍之间。在乙醇组和水对照组之间的差异中，有几个显著的生物学过程类别在两个区域之间是共同的（例如，突触传递、神经突发育）；然而，在这些类别中，两个区域之间的基因很少有共同之处。总体而言，这些结果表明，P 大鼠的 binge 样饮酒会导致 ACB-shell 和 CeA 中基因表达的区域依赖性变化，这些变化可能改变转录、突触功能和神经元可塑性；在每个区域内，这些改变的机制可能不同，因为 ACB-shell 和 CeA 之间很少有共同的乙醇反应基因。
