Institut National de Recherche Scientifique-Centre Eau Terre Environnement, Université du Québec, 490 de Couronne, 490 rue de Couronne, Québec, QC, Canada G1K 9A9.
Ecotoxicol Environ Saf. 2010 May;73(4):572-8. doi: 10.1016/j.ecoenv.2010.01.004. Epub 2010 Feb 8.
Early-life stages of fathead minnows were exposed to environmentally relevant concentrations of aqueous and dietary nickel and thallium and metal accumulation was monitored from the embryo until the larvae reached 21 days after hatching. During and after metal exposure, 6 toxicity endpoints were measured: time to hatch, embryo survival rate, routine metabolic rate and the activity of key enzymes (lactate dehydrogenase, nucleoside diphosphate kinase (NDPK), cytochrome C oxidase (CCO)). Although both Ni and Tl bioaccumulation were significant in embryos and non-feeding larvae, water was the major source of Ni and Tl in feeding larvae. Exposure to aqueous Ni decreased time to hatch and increased aerobic and biosynthetic capacities (as indicated by a higher activity of CCO and NDPK, respectively), suggesting that aqueous Ni exposure stimulates metabolism in early-life stages of fathead minnows.
早期阶段的翻车鱼被暴露在环境相关浓度的水和饮食镍和铊和金属积累进行监测，从胚胎直到幼虫孵化后 21 天。在金属暴露期间和之后，测量了 6 个毒性终点：孵化时间、胚胎存活率、常规代谢率和关键酶的活性（乳酸脱氢酶、核苷二磷酸激酶（NDPK）、细胞色素 C 氧化酶（CCO））。虽然 Ni 和 Tl 的生物积累在胚胎和非摄食幼虫中都很显著，但水是摄食幼虫中 Ni 和 Tl 的主要来源。暴露在水中的 Ni 会降低孵化时间，增加有氧和生物合成能力（分别表现为 CCO 和 NDPK 的活性更高），这表明水中的 Ni 暴露刺激了翻车鱼早期生命阶段的新陈代谢。
