Biotechnology Laboratory, NIDDK, NIH Bethesda, MD 20892, USA.
Curr Opin Biotechnol. 2010 Feb;21(1):21-6. doi: 10.1016/j.copbio.2010.01.001. Epub 2010 Jan 29.
The analysis of metabolic differences in bacterial strains is a useful tool for the development of strains with desired growth and production properties. Several methods are available for the evaluation and understanding of the differences: Biochemical methods to measure metabolites concentration and enzyme activity, mathematical methods to analyze metabolic fluxes through the various pathways, proteomic methods to identify expressed proteins, and genomic methods to detect and measure gene expression. A combination of the various methods is required to obtain a comprehensive understanding of metabolic activities. The genomic methods provide substantial amount information on global gene expression but do not always reflect the actual activity of the individual components. The review focuses on the different methodologies and their use, as well as historical overview of the evaluation of the differences between Escherichia coli K and E. coli B.
分析细菌菌株的代谢差异是开发具有理想生长和生产特性的菌株的有用工具。有几种方法可用于评估和理解这些差异：生化方法可用于测量代谢物浓度和酶活性，数学方法可用于分析通过各种途径的代谢通量，蛋白质组学方法可用于鉴定表达蛋白，基因组学方法可用于检测和测量基因表达。需要结合使用各种方法才能全面了解代谢活动。基因组学方法可提供大量有关全局基因表达的信息，但并不总是反映单个成分的实际活性。本文综述了不同的方法及其用途，以及对大肠杆菌 K 和大肠杆菌 B 之间差异的评估的历史概述。
