Escola Bahiana de Medicina e Saúde Pública - Dentistry Course, Salvador, Bahia, Brazil.
J Photochem Photobiol B. 2010 Mar 8;98(3):188-92. doi: 10.1016/j.jphotobiol.2009.12.005. Epub 2010 Jan 6.
Laser biomodulation has been getting considerable attention when it comes to its effects on the inflammatory process. Its action upon mast cells have been already studied, but none of the previous papers related the resulting effect to the inflammatory and vascular status of the wounds. Therefore, the acute inflammatory process as well as the mast cells behavior and the vascular response were analyzed under the influence of laser treatment on cutaneous wounds. Surgical procedures were performed on 60 rats divided into sham and laser groups. Low-level laser therapy was performed following surgical procedures (670 nm, 9 mW, 4 J/cm(2), 124 s). Histological specimens were analyzed for cell morphology and immunohistochemistry using anti-von Willebrand Factor and anti-Vascular Endothelial Growth Factor antibody. Laser treatment resulted in an increased acute inflammatory response in irradiated tissues; surgical wounds treated with laser therapy had increased polymorphonuclear cells, mast cells and vasodilation and lower numbers of vessels than those in control rats. Laser treatment resulted in higher expression of VEGF in irradiated tissues 6-24h post-treatment (p=0.002). It is possible to observe an amplification of acute inflammatory process during the first hours after surgical procedure in rats submitted to laser therapy.
激光生物调节在其对炎症过程的影响方面受到了相当大的关注。已经研究了其对肥大细胞的作用，但以前没有一篇论文将其结果与伤口的炎症和血管状态联系起来。因此，在激光处理对皮肤伤口的影响下，分析了急性炎症过程以及肥大细胞行为和血管反应。在 60 只大鼠上进行了手术程序，分为假手术和激光组。手术后进行低水平激光治疗（670nm，9mW，4J/cm(2)，124s）。使用抗血管性血友病因子和抗血管内皮生长因子抗体对组织学标本进行细胞形态和免疫组织化学分析。激光治疗导致照射组织中的急性炎症反应增加；与对照组大鼠相比，激光治疗的手术伤口中多形核细胞、肥大细胞和血管扩张增加，血管数量减少。激光治疗后 6-24 小时照射组织中 VEGF 的表达增加（p=0.002）。在接受激光治疗的大鼠中，在手术后的最初几小时内，可以观察到急性炎症过程的放大。
