Fathabadie Fateme Fadaie, Bayat Mohammad, Amini Abdolah, Bayat Maryam, Rezaie Fatemealsadat
Anatomy and Cell Biology Department, Shahid Beheshti University of Medical Sciences , Tehran , Iran.
J Cosmet Laser Ther. 2013 Dec;15(6):294-304. doi: 10.3109/14764172.2013.764435. Epub 2013 Mar 6.
Low-level laser therapy (LLLT) has been reported to be capable of changing mast cell numbers and degranulation in experimental wounds.
We conducted a study on the influence of pulsed LLLT on mast cells in wounds of non-diabetic and diabetic rats.
Thirty-six rats were randomly divided into non-diabetic and diabetic groups. Type 1 diabetes milletes (DM) was induced in rats of the diabetic group by administration of streptozotocin (STZ). We inflicted two wounds in each rat. The first wound in both non-diabetic and diabetic groups was treated with an 890-nm laser, having pulse frequency of 80 Hz and energy density of 0.2 J/cm(2). Mast cell numbers and degranulation of all subgroups were assessed at 4, 7, and 15 days after the infliction of the wounds.
According to the paired t-test, the total number of laser-treated mast cells was significantly higher than that of the placebos in the non-diabetic groups on days 4 and 15. The total number of laser-treated mast cells was significantly higher than that of the placebos in the diabetic groups on days 4 and 15. The number of granulated mast cells was significantly higher than that of degranulated mast cells for all laser-treated mast cells and placebo mast cells of the non-diabetic and diabetic groups.
据报道，低强度激光疗法（LLLT）能够改变实验性伤口中的肥大细胞数量和脱颗粒情况。
我们开展了一项关于脉冲式LLLT对非糖尿病和糖尿病大鼠伤口中肥大细胞影响的研究。
36只大鼠被随机分为非糖尿病组和糖尿病组。通过给糖尿病组大鼠注射链脲佐菌素（STZ）诱导1型糖尿病。在每只大鼠身上制造两处伤口。非糖尿病组和糖尿病组的第一个伤口均用波长890 nm、脉冲频率80 Hz、能量密度0.2 J/cm²的激光进行治疗。在伤口造成后的第4、7和15天评估所有亚组的肥大细胞数量和脱颗粒情况。
根据配对t检验，在第4天和第15天，非糖尿病组中接受激光治疗的肥大细胞总数显著高于安慰剂组。在第4天和第15天，糖尿病组中接受激光治疗的肥大细胞总数显著高于安慰剂组。对于非糖尿病组和糖尿病组中所有接受激光治疗的肥大细胞和安慰剂肥大细胞，颗粒化肥大细胞的数量显著高于脱颗粒肥大细胞的数量。
