[激光针刺镇痛与肥大细胞功能之间的关系]

[Relationship between laser acupuncture analgesia and the function of mast cells].

作者信息

Cheng Ke, Shen Xue-yong, Ding Guang-hong, Wu Fan

机构信息

Shanghai University of TCM, Shanghai 201203, China.

出版信息

Zhongguo Zhen Jiu. 2009 Jun;29(6):478-83.

PMID:19563196

Abstract

OBJECTIVE

To observe the analgesic effects of single-and combined-laser irradiation with low-intensity applied at "Zusanli" (ST 36) in rats, and their relation to degranulation of mast cells.

METHODS

Sixty-six SD rats were randomly divided into 6 groups: normal control group (Group NC), model control group (Group MC), sham irradiation group (Group SI), 10.6 microm laser irradiation group (Group 10.6 microm LI), 650 nm laser irradiation group (Group 650 nm LI) and combined (10.6 microm + 650 nm) laser irradiation group (Group CLI). Complete Freund's Adjuvant (0.05 mL) was injected into the left ankle joints of all the rats except those in Group NC to cause acute adjuvant-induced arthritis. In treatment, laser irradiation was applied at "Zusanli" (ST 36) for 30 minutes in all the rats except those in Group NC and Group MC. The paw withdrawal latency (PWL) to radian heat was used to compare analgesic effects among the groups. By means of toluidine blue, dyed slices of local tissues of "Zusanli" (ST 36) were used to observe changes of mast cell degranulation before and after laser irradiation.

RESULTS

The pain thresholds to irradiation of the rats in Group 650 nm LI and Group CLI were significantly higher than those in Group MC and Group SI (P < 0.01), and the mast cell degranulation rate in Group 650 nm LI and Group CLI were also significantly higher than that in Group MC and Group SI (P < 0.001). The pain threshold and mast cell degranulation rate in Group 10. 6 microm LI were not significantly different from those in Group MC and Group SI. There was a linear correlation between mast cell degranulation rate and PWL with 0. 737 in coefficient (P < 0.001).

CONCLUSION

Single 650 nm laser and combined 650 nm + 10.6 microm laser with low intensity irradiated at "Zusanli" (ST 36) in acute adjuvant rats can provide remarkable analgesic effects, and there was a positive correlation between mast cell degranulation rate and analgesic effects, which plays an important part in laser irradiation-induced analgesia.

摘要

目的

观察低强度单波长及联合波长激光照射大鼠“足三里”穴（ST 36）的镇痛效应及其与肥大细胞脱颗粒的关系。

方法

将66只SD大鼠随机分为6组：正常对照组（NC组）、模型对照组（MC组）、假照射组（SI组）、10.6μm激光照射组（10.6μm LI组）、650nm激光照射组（650nm LI组）和联合（10.6μm + 650nm）激光照射组（CLI组）。除NC组外，其余各组大鼠左踝关节注射完全弗氏佐剂0.05 mL，制备急性佐剂性关节炎模型。治疗时，除NC组和MC组外，其余各组大鼠均于“足三里”穴（ST 36）进行激光照射30分钟。采用辐射热刺激法测定大鼠 paw withdrawal latency（PWL），比较各组镇痛效果。采用甲苯胺蓝染色法观察激光照射前后“足三里”穴（ST 36）局部组织切片肥大细胞脱颗粒变化。

结果

650nm LI组和CLI组大鼠照射痛阈显著高于MC组和SI组（P < 0.01），650nm LI组和CLI组肥大细胞脱颗粒率也显著高于MC组和SI组（P < 0.001）。10.6μm LI组痛阈和肥大细胞脱颗粒率与MC组和SI组比较，差异无统计学意义。肥大细胞脱颗粒率与PWL呈线性相关，相关系数为0.737（P < 0.001）。