Laboratory of Fish Immunology and Genetics, The Mina and Everard Goodman Faculty of Life Sciences, Bar-Ilan University, Ramat-Gan 52900, Israel.
Virology. 2010 Apr 10;399(2):239-47. doi: 10.1016/j.virol.2010.01.001. Epub 2010 Feb 1.
We report here an outbreak of an acute disease that caused high mortality rate in laboratory-reared tilapia larvae. The disease was initially observed in inbred gynogenetic line of blue tilapia larvae (Oreochromis aureus) and could be transmitted to larvae of other tilapia species. Based on the clinical manifestation (a whirling syndrome), we refer to the disease as viral encephalitis of tilapia larvae. The disease-associated DNA virus is described and accordingly designated tilapia larvae encephalitis virus (TLEV). A primary morphological, biophysical and molecular characterization of TLEV is presented. By virtue of these properties, the newly discovered virus is a herpes-like virus. Phylogenetic analysis, albeit limited, confirms this assumption and places TLEV within the family of Herpesviridae and distantly from the families Alloherpesviridae and Iridoviridae. By using PCR with virus-specific primers, diseased larvae and adult TLEV carriers were also identified in tilapia delivered from external hatcheries.
我们在此报告一起在实验室养殖的罗非鱼幼鱼中爆发的一种急性疾病，该病死亡率很高。该病最初出现在蓝罗非鱼（奥利亚罗非鱼）的近交雌核发育系幼鱼中，可传播给其他罗非鱼物种的幼鱼。根据临床表现（旋转综合征），我们将该病称为罗非鱼幼鱼脑炎。对与疾病相关的 DNA 病毒进行了描述，并将其命名为罗非鱼幼鱼脑炎病毒（TLEV）。本文介绍了 TLEV 的初步形态、生物物理和分子特征。凭借这些特性，新发现的病毒是一种疱疹样病毒。尽管有限，但系统发育分析证实了这一假设，并将 TLEV 置于疱疹病毒科内，与 Alloherpesviridae 和 Iridoviridae 科较远。使用针对病毒的特异性引物的 PCR 方法，还在从外部孵化场运送的罗非鱼中鉴定出了患病的幼鱼和携带 TLEV 的成年鱼。
