First Neurosurgery Clinic, Diskapi Yildirim Beyazit Education and Research Hospital, Ankara, Turkey.
J Clin Neurosci. 2010 Apr;17(4):486-9. doi: 10.1016/j.jocn.2009.07.108. Epub 2010 Feb 1.
Although the precise mechanism by which ischemia/reperfusion injury occurs in the spinal cord remains unclear, it is evident that free oxygen radicals and apoptosis play major roles in the destruction of membrane lipids, damage to DNA and cell death. The apoptotic process involves activation of the caspase-3 cascade. Although it is widely used as a protective agent against cell injury, it is unknown whether mesna (2-mercaptoethane sulfonate) ameliorates neuronal ischemic injury. The aim of this study was to determine the effect of mesna on caspase-3 activity in a rabbit model. Adult rabbits underwent spinal cord ischemic injury via occlusion of the abdominal aorta for 20 min. Twenty-four hours after ischemia, spinal cord samples were obtained and tissue caspase-3 activity was measured. Rabbits that had been given a single dose of 150 mg/kg mesna had decreased caspase-3 activity in the spinal cord following ischemia/reperfusion injury, indicating a protective effect. However, caspase-3 activity was lower in rabbits given methylprednisolone than in those given mesna, indicating that methylprednisolone has the stronger protective effect of the two agents.
尽管脊髓缺血再灌注损伤的确切机制仍不清楚，但很明显，游离氧自由基和细胞凋亡在破坏膜脂质、损伤 DNA 和细胞死亡方面起着重要作用。凋亡过程涉及 caspase-3 级联的激活。虽然它被广泛用作细胞损伤的保护剂，但尚不清楚美司钠（2-巯基乙烷磺酸钠）是否能改善神经元缺血性损伤。本研究旨在确定美司钠对兔模型中 caspase-3 活性的影响。成年兔通过阻断腹主动脉 20 分钟来进行脊髓缺血性损伤。缺血 24 小时后，获取脊髓样本并测量组织 caspase-3 活性。在缺血/再灌注损伤后，单次给予 150mg/kg 美司钠的兔子脊髓 caspase-3 活性降低，表明具有保护作用。然而，给予甲基强的松龙的兔子的 caspase-3 活性低于给予美司钠的兔子，表明甲基强的松龙这两种药物具有更强的保护作用。
