Mayo Clinic and Foundation, Rochester, MN 55905, USA.
Cytokine. 2010 Apr;50(1):24-9. doi: 10.1016/j.cyto.2009.12.002. Epub 2010 Feb 1.
We conducted a population-based study on 738 schoolchildren who received two doses of rubella vaccine in order to determine cytokine secretion patterns and their associations with demographic and clinical variables. The results showed a robust rubella-specific inflammatory cytokine response characterized by high median [inter-quartile range (IQR)] secretion levels (in pg/mL) of IL-6 [3681.0 (3160.0, 4052.0)], GM-CSF [28.0 (23.6, 32.6)], and TNF-alpha [29.7 (-7.0, 89.2)]. We also detected modest levels of rubella-specific secretion of Th1 cytokines IL-2 and IFN-gamma, while IL-12p40 was undetectable. In contrast, rubella-specific Th2 responses were hardly detectable. Age at vaccination, enrollment, and time elapsed between last vaccination and enrollment was significantly associated with the outcome of IL-2, IL-6, and IFN-gamma secretion. These results suggest an immune-deviation or "skewing" from Th1/Th2 cytokine patterns towards a predominant inflammatory response upon in vitro rubella virus stimulation.
我们对 738 名接受两剂风疹疫苗的学童进行了一项基于人群的研究，以确定细胞因子分泌模式及其与人口统计学和临床变量的关系。结果显示，风疹特异性炎症细胞因子反应强烈，表现为 IL-6[3681.0（3160.0，4052.0）]、GM-CSF[28.0（23.6，32.6）]和 TNF-α[29.7（-7.0，89.2）]的中位数[四分位距（IQR）]分泌水平较高。我们还检测到风疹特异性 Th1 细胞因子 IL-2 和 IFN-γ的适度分泌，而 IL-12p40 则无法检测到。相比之下，风疹特异性 Th2 反应几乎无法检测到。疫苗接种时的年龄、入组时的年龄以及最后一次接种和入组之间的时间间隔与 IL-2、IL-6 和 IFN-γ分泌的结果显著相关。这些结果表明，在体外风疹病毒刺激下，免疫偏离或“偏向”Th1/Th2 细胞因子模式，偏向于主要的炎症反应。
