Department of Small Animal Medicine and Surgery, School of Veterinary Medicine Hannover, Bischofsholer Damm 15, 30173 Hannover, Germany.
Vet J. 2011 Feb;187(2):260-6. doi: 10.1016/j.tvjl.2009.12.001. Epub 2010 Feb 1.
Canine steroid-responsive meningitis-arteritis (SRMA) is a systemic inflammatory disease with a predominant manifestation within the cervical meninges, increased immunoglobulin A (IgA) levels in serum and cerebrospinal fluid (CSF), and a shift of the B:T cell ratio towards a higher percentage of B cells. A Th2-dominated immune response associated with SRMA was therefore hypothesised. Pellets of peripheral blood mononuclear cells (PBMNCs) and CSF white blood cells (CSF WBCs) from dogs in the acute phase of SRMA (n=16) and under glucocorticoid treatment for SRMA (n=16) were investigated for interleukin (IL)-2, interferon (IFN)-γ, IL-4, IL-5 and IL-10 mRNA expression by means of reverse-transcriptase real-time polymerase chain reaction. Results were compared with those of dogs with other inflammatory (n=9) and neoplastic disorders (n=10) of the central nervous system. A tendency towards low levels of Th1 response related cytokines (IL-2, IFN-γ) and high IL-4 expression was observed indicating a Th2-skewed immune response. The pronounced IL-4 production may be an important pathogenetic factor for excessive IgA production in the acute phase of SRMA and for those cases under glucocorticoid treatment.
犬类固醇反应性脑膜炎-动脉炎（SRMA）是一种系统性炎症性疾病，主要表现为颈部脑膜受累，血清和脑脊液（CSF）中免疫球蛋白 A（IgA）水平升高，B:T 细胞比例向更高比例的 B 细胞转移。因此，假设与 SRMA 相关的是 Th2 占主导地位的免疫反应。对处于 SRMA 急性期（n=16）和接受糖皮质激素治疗的 SRMA（n=16）犬的外周血单个核细胞（PBMNC）和 CSF 白细胞（CSF WBC）颗粒进行白细胞介素（IL）-2、干扰素（IFN）-γ、IL-4、IL-5 和 IL-10mRNA 表达的逆转录实时聚合酶链反应。将结果与患有其他中枢神经系统炎症（n=9）和肿瘤性疾病（n=10）的犬进行比较。观察到与 Th1 反应相关的细胞因子（IL-2、IFN-γ）水平降低和 IL-4 表达升高的趋势，表明存在 Th2 偏向的免疫反应。明显的 IL-4 产生可能是 SRMA 急性期过度 IgA 产生和那些接受糖皮质激素治疗的病例的重要发病机制因素。
