Cheng Jing, Wu Min, Wu Zhong-hai
Department of Physiology, Southern Medical University, Guangzhou 510515, China.
Nan Fang Yi Ke Da Xue Xue Bao. 2010 Jan;30(1):51-3.
To observe the effect of glycine on the discharge activities of inspiratory neurons in the medulla oblongata slices of neonatal rats.
Neonatal rat medulla oblongata slices including the medial region of the nucleus retrofacialis (mNRF) with the hypoglossal nerve rootlets retained were prepared. The activity of the inspiratory neurons in the mNRF and the respiratory rhythmical discharge activity (RRDA) of the hypoglossal nerve rootlets were simultaneously recorded using microelectrodes and suction electrodes, respectively. The effect of glycine on the rhythmical respiration was investigated by administration of glycine receptor agonist and its specific antagonist strychnine (STR) into a modified Kreb's perfusion solution (MKS).
Glycine significantly shortened the inspiratory time (TI) and integral amplitude (IA) of the inspiratory neurons and decreased the peak frequency (PFn). By contrast, STR induced a decrease in the expiratory time (TE) and respiratory cycle (RC) of the inspiratory neurons, but the peak frequency was not significantly affected.
Glycine and its receptor play a role in the modulation of the rhythmical respiration, and glycine is probably involved in the phase-switching between expiration and inspiration by affecting the inspiratory neurons.
观察甘氨酸对新生大鼠延髓切片中吸气神经元放电活动的影响。
制备保留舌下神经根丝的包含面神经后核内侧区域（mNRF）的新生大鼠延髓切片。分别使用微电极和吸力电极同时记录mNRF中吸气神经元的活动以及舌下神经根丝的呼吸节律性放电活动（RRDA）。通过向改良的克雷布斯灌注液（MKS）中加入甘氨酸受体激动剂及其特异性拮抗剂士的宁（STR），研究甘氨酸对节律性呼吸的影响。
甘氨酸显著缩短了吸气神经元的吸气时间（TI）和积分幅度（IA），并降低了峰值频率（PFn）。相比之下，STR导致吸气神经元的呼气时间（TE）和呼吸周期（RC）缩短，但峰值频率未受到显著影响。
甘氨酸及其受体在节律性呼吸的调节中发挥作用，甘氨酸可能通过影响吸气神经元参与呼气和吸气之间的相位转换。
