Qian Zhi-bin, Qi Ying, Wu Zhong-hai
Department of Physiology, Southern Medical University, Guangzhou 510515, China.
Nan Fang Yi Ke Da Xue Xue Bao. 2010 Jan;30(1):54-6.
To investigate the role of histamine H1 receptors in modulating the discharge activities of the inspiratory neurons in the nucleus retrofacialis of neonatal rats.
Brainstem slices were obtained from neonatal rats containing the medial region of the nucleus retrofacialis (mNRF) with the hypoglossal nerve (XII nerve) rootlets retained. The rhythmic discharges of the inspiratory neurons (I neurons) and activities of the XII nerve rootlets were simultaneously recorded using microelectrodes and suction electrodes, respectively. The role of H1 receptors in modulation of the discharge activities of the inspiratory neurons was investigated using the H1 receptor agonist histamine and its specific antagonist pyrilamine dissolved in modified Kreb's solution for slice perfusion.
Histamine shortened the respiratory cycle (RC) and expiratory time (TE) of the neurons in the brain slices, and pyrilamine produced the opposite effects. Neither histamine nor pyrilamine affected the inspiratory time (TI), integral amplitude (IA) or the peak discharge frequency (PF) of the I neurons.
H1 receptors play an excitatory role in the modulation of the discharge activities of the inspiratory neurons in neonatal rat brainstem slices.
研究组胺H1受体在调节新生大鼠面神经后核吸气神经元放电活动中的作用。
从新生大鼠获取脑干切片，保留含面神经后核内侧区域（mNRF）及舌下神经根丝。分别使用微电极和吸力电极同时记录吸气神经元（I神经元）的节律性放电和舌下神经根丝的活动。通过将H1受体激动剂组胺及其特异性拮抗剂吡拉明溶解于改良的Krebs溶液中进行脑片灌流，研究H1受体对吸气神经元放电活动的调节作用。
组胺缩短了脑片中神经元的呼吸周期（RC）和呼气时间（TE），而吡拉明则产生相反的作用。组胺和吡拉明均不影响I神经元的吸气时间（TI）、积分幅度（IA）或放电峰值频率（PF）。
H1受体在调节新生大鼠脑干切片中吸气神经元的放电活动中起兴奋作用。
