考拉维酮（来自可乐果种子的双黄酮）对γ射线照射的 Wistar 白化大鼠大脑的保护作用。
Protective effect of kolaviron, a biflavonoid from Garcinia kola seeds, in brain of Wistar albino rats exposed to gamma-radiation.
机构信息
Drug Metabolism and Toxicology Research Laboratories, Department of Biochemistry, College of Medicine, Faculty of Basic Medical Sciences, University of Ibadan, Ibadan-20005, Nigeria.
出版信息
Biol Pharm Bull. 2010;33(2):260-6. doi: 10.1248/bpb.33.260.
This study was designed to evaluate the protective effect of kolaviron (KV), a biflavonoid from Garcinia kola seeds, against gamma-radiation (5 Gy)-induced oxidative stress in brain of Wistar rats. Vitamin C (VC) served as standard antioxidant. Forty-four rats were divided into 4 groups of 11 animals each. One group was un-irradiated (normal), two groups were treated with KV and VC (250 mg/kg) for 6 weeks prior to and 8 weeks after irradiation, and fourth group was only irradiated. Rats were sacrificed 1 and 8 weeks after irradiation. Cellular alterations were monitored using changes in the levels of malondialdehyde (MDA)-an index of lipid peroxidation, superoxide dismutase (SOD), glutathione-S-transferase (GST), reduced glutathione (GSH), catalase (CAT), alanine and aspartate aminotransferases (ALT and AST), urea and creatinine. MDA levels increased significantly (p<0.05) by 90% and 151% after 1 and 8 weeks of irradiation. Furthermore, levels of GSH and antioxidant enzymes were significantly (p<0.05) decreased in gamma-irradiated animals. GSH and GST decreased by 61% and 43% after 1 week, and by 75% and 74%, after 8 weeks of exposure, respectively. gamma-Irradiation decreased SOD and CAT levels by 53% and 68%, respectively, and caused significant (p<0.05) increases in serum ALT, AST and urea after 8 weeks of exposure. Treatment with KV and VC significantly decreased the levels of MDA, ALT, AST and urea. The antioxidant indices were significantly ameliorated in KV-treated animals. These data suggest that kolaviron may protect against gamma-radiation-induced oxidative stress in brain of exposed rats.
本研究旨在评估 kolaviron（来自可乐果种子的双黄酮）对 Wistar 大鼠脑辐射（5 Gy）诱导的氧化应激的保护作用。维生素 C（VC）用作标准抗氧化剂。将 44 只大鼠分为 4 组，每组 11 只。一组未照射（正常），两组在照射前和照射后 6 周用 KV 和 VC（250 mg/kg）治疗，第四组仅照射。照射后 1 周和 8 周处死大鼠。通过丙二醛（MDA）水平的变化来监测细胞变化 - 脂质过氧化的指标，超氧化物歧化酶（SOD），谷胱甘肽-S-转移酶（GST），还原型谷胱甘肽（GSH），过氧化氢酶（CAT），丙氨酸和天冬氨酸氨基转移酶（ALT 和 AST），尿素和肌酐。照射后 1 周和 8 周 MDA 水平分别显着增加（p<0.05）90％和 151％。此外，γ 照射动物的 GSH 和抗氧化酶水平显着降低（p<0.05）。GSH 和 GST 在 1 周后分别下降 61％和 43％，在 8 周后分别下降 75％和 74％。γ 照射使 SOD 和 CAT 水平分别降低 53％和 68％，并在 8 周暴露后导致血清 ALT，AST 和尿素显着增加（p<0.05）。用 KV 和 VC 治疗显着降低了 MDA，ALT，AST 和尿素的水平。在 KV 处理的动物中，抗氧化指标得到显着改善。这些数据表明，kolaviron 可能对暴露于辐射的大鼠的脑辐射诱导的氧化应激具有保护作用。
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