Fujimoto Nariaki, Baurzhan Arailym, Chaizhunusova Nailya, Amantayeva Gaukhar, Kairkhanova Ynkar, Shabdarbaeva Dariya, Zhunussov Yersin, Zhumadilov Kassym, Stepanenko Valeriy, Gnyrya Vyacheslav, Azhimkhanov Almas, Kolbayenkov Alexander, Hoshi Masaharu
Research Institute for Radiation Biology and Medicine, Hiroshima University, Hiroshima, Japan.
Semey Medical University, Semey, Kazakhstan.
Eurasian J Med. 2020 Feb;52(1):52-56. doi: 10.5152/eurasianjmed.2020.19228.
The pathological effects of internal exposure to manganese dioxide-56 (MnO) radioisotope particles have been previously examined in rats. Here we further examine the effects of MnO, focusing on changes in blood parameters.
Ten-week-old male Wistar rats were exposed to 3 doses of neutron-activated MnO powder, nonradioactive MnO powder, or external 60Co γ-rays (1 Gy, whole body). On days 3 and 61 postexposure, the animals were necropsied to measure organ weights and clinical blood parameters, including red blood cell and white blood cell counts; concentrations of calcium, phosphorus, potassium, and sodium; and levels of alanine aminotransferase (ALT), aspartate aminotransferase, amylase, creatinine, urea, total protein, albumin, triglycerides, high density lipoprotein, total cholesterol, and glucose.
In the MnO-exposed animals, accumulated doses were found to be highest in the gastrointestinal tract, followed by the skin and lungs, with whole-body doses ranging from 41 to 100 mGy. There were no MnO exposure-related changes in body weights or relative organ weights. The ALT level decreased on day 3 and then significantly increased on day 61 in the MnO-exposed groups. There were no exposure-related changes in any other blood parameters.
Although the internal doses were less than 100 mGy, internal exposure of MnO powder showed significant biological impacts.
此前已在大鼠中研究了二氧化锰 - 56（MnO）放射性同位素颗粒内照射的病理效应。在此，我们进一步研究MnO的效应，重点关注血液参数的变化。
将10周龄雄性Wistar大鼠暴露于3种剂量的中子活化MnO粉末、非放射性MnO粉末或外部60Coγ射线（1 Gy，全身照射）。在暴露后第3天和第61天，对动物进行剖检以测量器官重量和临床血液参数，包括红细胞和白细胞计数；钙、磷、钾和钠的浓度；以及丙氨酸氨基转移酶（ALT）、天冬氨酸氨基转移酶、淀粉酶、肌酐、尿素、总蛋白、白蛋白、甘油三酯、高密度脂蛋白、总胆固醇和葡萄糖水平。
在暴露于MnO的动物中，胃肠道中的累积剂量最高，其次是皮肤和肺，全身剂量范围为41至100 mGy。暴露于MnO的动物体重或相对器官重量没有与MnO暴露相关的变化。在暴露于MnO的组中，ALT水平在第3天下降，然后在第61天显著升高。其他血液参数没有与暴露相关的变化。
尽管内照射剂量小于100 mGy，但MnO粉末的内照射显示出显著的生物学影响。
