过氧化物酶体增殖物激活受体 α（PPARα）刺激大鼠心肌的蛋白质组学分析。
Proteomics analysis of cardiac muscle from rats with peroxisomal proliferator-activated receptor alpha (PPARalpha) stimulation.
机构信息
ActivX Biosciences Inc., La Jolla, CA 92037, USA.
出版信息
J Toxicol Sci. 2010 Feb;35(1):131-5. doi: 10.2131/jts.35.131.
To investigate peroxisomal proliferator-activated receptor alpha (PPARalpha) signal responses in heart muscle, we performed LC-MS/MS-based proteomics analysis of heart muscle from rats given fenofibrate or clofibrate. Fenofibrate increased the expression of ACAA2, DECR1, and ECH1 consistent with activation of PPARalpha. Fenofibrate and clofibrate reduced the expression of 10 and 12 proteins, respectively with the expression of ACSL1, SLC25A4, A1BG, HADHA, ATP2A2, BDH1, ETFDH, HADHB, and CPT2 being reduced in common with both of fibrate-treated groups. The approach adopted in this study provides an efficient method for monitoring global changes in protein expression.
为了研究过氧化物酶体增殖物激活受体α (PPARα) 在心肌中的信号反应，我们对给予非诺贝特或氯贝丁酯的大鼠的心肌进行了基于 LC-MS/MS 的蛋白质组学分析。非诺贝特增加了 ACAA2、DECR1 和 ECH1 的表达，与 PPARα 的激活一致。非诺贝特和氯贝丁酯分别降低了 10 种和 12 种蛋白质的表达，其中 ACSL1、SLC25A4、A1BG、HADHA、ATP2A2、BDH1、ETFDH、HADHB 和 CPT2 的表达在两种纤维酸处理组中均降低。本研究中采用的方法为监测蛋白质表达的全局变化提供了一种有效的方法。
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