Cranfield Health, Cranfield University, Bedford MK43 0AL, United Kingdom.
Pediatr Res. 2010 May;67(5):476-80. doi: 10.1203/PDR.0b013e3181d61c0c.
The rise of antibiotic-resistant bacteria is a major concern for the continued health and well-being of the general population. The widespread use of antibiotics within the farming industry is one of the factors that have been linked to the appearance of these resistant strains. Regulation exists to prevent antibiotic foodstuffs such as milk being distributed to consumers; however, for this to be effective, methods must exist for testing the milk to enforce these regulations. Current techniques are often time consuming and expensive, especially when applied to large number of samples. This has led to an interest in milk analyses, which can be carried out outside the laboratory. The development of immunosensors that exploit the exquisite specificity of antibody binding has been studied, both using labeled antibodies such as ELISA assays and, more recently, label-free approaches that directly detect the presence of the antibiotic on binding to a specific antibody. Within this review, we detail recent advances in the detection of antibiotics, especially in milk, using a variety of methods. Various techniques such as electrochemical and surface plasmon resonance methods are described.
抗生素耐药菌的出现是广大民众健康和福祉持续面临的主要问题之一。农业领域抗生素的广泛使用是出现这些耐药菌株的因素之一。存在相关规定来防止抗生素食品（如牛奶）分发给消费者；然而，为了使这些规定有效，必须存在测试牛奶的方法来执行这些规定。目前的技术通常既耗时又昂贵，尤其是在处理大量样本时。这导致人们对可以在实验室外进行的牛奶分析产生了兴趣。已经研究了利用抗体结合的高度特异性来开发免疫传感器，包括使用标记抗体（如 ELISA 测定）和最近的直接检测抗生素与特定抗体结合时存在的无标记方法。在本综述中，我们详细介绍了使用各种方法检测抗生素（特别是牛奶中的抗生素）的最新进展。描述了各种技术，如电化学和表面等离子体共振方法。
