Myology Group, UMR S 787 INSERM, Université Pierre et Marie Curie Paris VI, Paris, 75634, France.
Nat Cell Biol. 2010 Mar;12(3):257-66. doi: 10.1038/ncb2025. Epub 2010 Jan 31.
Satellite cells are resident myogenic progenitors in postnatal skeletal muscle involved in muscle postnatal growth and adult regenerative capacity. Here, we identify and describe a population of muscle-resident stem cells, which are located in the interstitium, that express the cell stress mediator PW1 but do not express other markers of muscle stem cells such as Pax7. PW1(+)/Pax7(-) interstitial cells (PICs) are myogenic in vitro and efficiently contribute to skeletal muscle regeneration in vivo as well as generating satellite cells and PICs. Whereas Pax7 mutant satellite cells show robust myogenic potential, Pax7 mutant PICs are unable to participate in myogenesis and accumulate during postnatal growth. Furthermore, we found that PICs are not derived from a satellite cell lineage. Taken together, our findings uncover a new and anatomically identifiable population of muscle progenitors and define a key role for Pax7 in a non-satellite cell population during postnatal muscle growth.
卫星细胞是成体骨骼肌中的常驻成肌祖细胞，参与肌肉的出生后生长和成年再生能力。在这里，我们鉴定并描述了一群位于间质中的肌肉驻留干细胞，它们表达细胞应激介质 PW1，但不表达其他肌肉干细胞标志物，如 Pax7。PW1(+) / Pax7(-) 间质细胞 (PICs) 在体外具有成肌能力，并能有效地促进体内骨骼肌再生，以及生成卫星细胞和 PICs。虽然 Pax7 突变的卫星细胞显示出强大的成肌潜能，但 Pax7 突变的 PIC 不能参与成肌作用，并在出生后生长过程中积累。此外，我们发现 PIC 不是来源于卫星细胞谱系。总之，我们的发现揭示了一种新的、可解剖识别的肌肉祖细胞群体，并定义了 Pax7 在出生后肌肉生长过程中在非卫星细胞群体中的关键作用。
