Uike N, Inazawa J, Nishigaki H, Takahira H, Katsuno M, Hashimoto M, Kozuru M
Department of Hematology, National Kyushu Cancer Center Hospital, Fukuoka, Japan.
Acta Haematol. 1991;85(1):51-5. doi: 10.1159/000204855.
In a case of Philadelphia chromosome (Ph1)-negative chronic myeloid leukemia (CML) without the Y chromosome, we investigated the differences, at the molecular level, from Ph1-positive CML. Using Southern blot analysis and in situ hybridization studies, we could demonstrate a rearrangement within the breakpoint cluster region (bcr), and the location of a bcr-abl fusion gene on chromosome 22. To our knowledge, this is the first case of Ph1-negative CML with a loss of the Y chromosome in which the molecular abnormalities are shown to be identical with those in Ph1-positive CML.
在一例无Y染色体的费城染色体（Ph1）阴性慢性粒细胞白血病（CML）中，我们在分子水平上研究了其与Ph1阳性CML的差异。通过Southern印迹分析和原位杂交研究，我们能够证实断点簇区域（bcr）内的重排以及22号染色体上bcr-abl融合基因的位置。据我们所知，这是首例无Y染色体的Ph1阴性CML病例，其分子异常显示与Ph1阳性CML相同。
