Dreazen O, Klisak I, Rassool F, Goldman J M, Sparkes R S, Gale R P
Department of Medicine, UCLA School of Medicine 90024.
Oncogene Res. 1988;2(2):167-75.
The relationship between chronic myelogenous leukemia (CML) with and without the Ph1 chromosome is controversial. Although some suggest that these disorders are identical, other reports suggest that Ph1 chromosome negative CML is a distinct entity. To resolve this issue, we studied 11 patients with Ph1 chromosome negative CML for the translocation of the Abelson proto-oncogene (c-abl) to the breakpoint cluster region gene (bcr), internal genomic rearrangement of bcr, and transcription of a chimeric bcr/abl mRNA. Our data indicate that c-abl is translocated to chromosome 22 where it is inserted after exon "2" or "3" of the bcr gene. This results in transcription of a chimeric bcr/abl mRNA in which the splice is between bcr exon "2" or "3" and c-abl exon 2. These data suggest that CML with and without the Ph1 chromosome are molecularly identical disorders with regard to bcr and abl.
伴有或不伴有Ph1染色体的慢性粒细胞白血病（CML）之间的关系存在争议。尽管一些人认为这些疾病是相同的，但其他报告表明Ph1染色体阴性的CML是一种独特的实体。为了解决这个问题，我们研究了11例Ph1染色体阴性的CML患者，检测了阿贝尔森原癌基因（c-abl）到断裂点簇区域基因（bcr）的易位、bcr的内部基因组重排以及嵌合bcr/abl mRNA的转录情况。我们的数据表明，c-abl易位至22号染色体，插入到bcr基因外显子“2”或“3”之后。这导致了嵌合bcr/abl mRNA的转录，其剪接发生在bcr外显子“2”或“3”与c-abl外显子2之间。这些数据表明，伴有或不伴有Ph1染色体的CML在bcr和abl方面是分子层面相同的疾病。
