McGilvray Kirk C, Santoni Brandon G, Turner A Simon, Bogdansky Simon, Wheeler Donna L, Puttlitz Christian M
Orthopaedic Bioengineering Research Laboratory, Department of Mechanical Engineering and School of Biomedical Engineering, Colorado State University, 1374 Campus Delivery, Fort Collins, CO 80523-1374, USA.
Cell Tissue Bank. 2011 May;12(2):89-98. doi: 10.1007/s10561-010-9170-z. Epub 2010 Jan 30.
Gamma radiation is established as a procedure for inactivating bacteria, fungal spores and viruses. Sterilization of soft tissue allografts with high dose (60)Co gamma radiation has been shown to have adverse effects on allograft biomechanical properties. In the current study, bone-patellar tendon-bone (BPTB) allografts from 32 mature sheep were divided into two treatment groups: low-dose radiation at 15 kGy (n = 16) and high-dose radiation at 25 kGy (n = 16) with the contralateral limb serving as a 0 kGy (n = 32) non-irradiated control. Half of the tendons from all treatment groups were biomechanically tested to determine bulk BPTB mechanical properties, cancellous bone compressive properties, and interference screw pull-out strength. The remaining tissues were prepared, implanted, and mechanically tested in an acute in vitro anterior crucial ligament (ACL) reconstruction. Low-dose radiation did not adversely affect mechanical properties of the tendon allograft, bone, or ACL reconstruction compared to internal non-irradiated control. However, high-dose radiation compromised bulk tendon load at failure and ultimate strength by 26.9 and 28.9%, respectively (P < 0.05), but demonstrated no negative effect on the cancellous bone compressive properties or interference screw pull-out strength. Our findings suggest that low dose radiation (15 kGy) does not compromise the mechanical integrity of the allograft tissue, yet high dose radiation (25 kGy) significantly alters the biomechanical integrity of the soft tissue constituent.
伽马辐射已被确立为一种灭活细菌、真菌孢子和病毒的方法。高剂量（60）钴伽马辐射对软组织同种异体移植物进行灭菌已被证明会对同种异体移植物的生物力学性能产生不利影响。在本研究中，将32只成年绵羊的骨-髌腱-骨（BPTB）同种异体移植物分为两个治疗组：15 kGy的低剂量辐射组（n = 16）和25 kGy的高剂量辐射组（n = 16），对侧肢体作为0 kGy（n = 32）的未辐照对照组。对所有治疗组的一半肌腱进行生物力学测试，以确定BPTB整体力学性能、松质骨压缩性能和干涉螺钉拔出强度。对其余组织进行制备、植入，并在急性体外前交叉韧带（ACL）重建中进行力学测试。与内部未辐照对照组相比，低剂量辐射对肌腱同种异体移植物、骨或ACL重建的力学性能没有不利影响。然而，高剂量辐射使肌腱在失效时的整体负荷和极限强度分别降低了26.9%和28.9%（P < 0.05），但对松质骨压缩性能或干涉螺钉拔出强度没有负面影响。我们的研究结果表明，低剂量辐射（15 kGy）不会损害同种异体移植物组织的机械完整性，而高剂量辐射（25 kGy）会显著改变软组织成分的生物力学完整性。
