Theil K W, Bohl E H, Agnes A G
Am J Vet Res. 1977 Nov;38(11):1765-8.
Two isolates of porcine rotavirus (reovirus-like agent) were isolated and passaged in primary procine kidney cell cultures. Viral infectivity for cells was monitored by immunofluorescence because viral cytopathic effect was moderate. Successful passage of virus in cell culture required that viral suspensions obtained from infected cell cultures be treated with pancreatin prior to inoculation onto cell monolayers. Porcine rotavirus passage in cell culture also was accomplished, using trypsin treatments in lieu of pancreatin treatments. Porcine rotavirus passaged 10 times in cell culture infected gnotobiotic pigs and caused diarrhea. Gnotobiotic pigs that recovered from this infection were resistant to challenge exposure with porcine rotavirus but were susceptible to challenge exposure with transmissible gastroenteritis virus. As determined by immunofluorescent cross reactions, porcine rotavirus was found to be antigenically related to the human and bovine rotaviruses but not to reovirus type 3 or to transmissible gastroenteritis virus.
分离出两株猪轮状病毒（呼肠孤病毒样因子），并在原代猪肾细胞培养物中传代。由于病毒致细胞病变效应中等，因此通过免疫荧光监测细胞的病毒感染性。病毒在细胞培养物中成功传代要求从感染细胞培养物中获得的病毒悬液在接种到细胞单层之前用胰蛋白酶处理。使用胰蛋白酶处理代替胰酶处理也实现了猪轮状病毒在细胞培养物中的传代。在细胞培养物中传代10次的猪轮状病毒感染了无菌猪并引起腹泻。从这种感染中恢复的无菌猪对猪轮状病毒的攻击暴露具有抵抗力，但对传染性胃肠炎病毒的攻击暴露易感。通过免疫荧光交叉反应确定，发现猪轮状病毒与人和牛轮状病毒在抗原上相关，但与3型呼肠孤病毒或传染性胃肠炎病毒无关。
